胆汁酸对肝星状细胞增殖及细胞动能的调控作用_英文_.pdfVIP

· · 临床儿科杂志 第 卷第 期 年 月 301 28 4 2010 4 J Clin Pediatr Vol．28 No．4 Apr ． 2010 · · Original Article in English Bile acids enhance proliferation and motility of hepatic stellate cell through regulation of p38 ／ JNK signaling ZHANG Yi-ning1 Tadashi Ikegami2 DU Hong-wei1 Yasushi Matsuzaki2 （ ， ， ）； 1． First Hospital of Jilin University Changchun 130021 Jilin China 2． Division of Gastroenterology ， （ ， ） and Hepatology Tokyo Medical University Kasumigaura Hospital Tsukuba 300－0395 Japan Abstract ： Objective Bile acids （BA ） facilitate cholesterol hepatic fibrosis． Although hepatic stellate cell （HSC ） is one of the most important cells during liver fibrogenesis ， the effect of bile acids on HSC is rarely mentioned． Therefore ， bile acids facilitate liver fibrosis through regulating activated HSC should be tested． Methods The amount of BrdU incor- poration was determined to assess the proliferation of HSC treated by bile acid． Wound-healing assay was used to determine the cellular motility． Meanwhile ， the phosphorylation of p38 and JNK in HSC was detected by Western blotting． Results 50 μmol ／ L GCDCA enhanced the HSC proliferation significantly （152．0％ ± 7．1％ ， P ＜ 0．05 ）； 50 μmol ／ L GCDCA also induced phosphorylation of p38 and JNK （450．0％ ± 12．2％ of control in p38 （P ＜ 0．01 ）， 210．0％ ± 1