MyD88抑制乙型肝炎病毒复制依赖活化NF-κB信号通路.pdfVIP

丝生塑皇蹙鎏!Q盟生!旦笠!鲞筮!塑』里婴垄垡丛!!!垣!!迥垒垂煎婴：玉些!Q鲤：型!：№：； MyD88抑制乙型肝炎病毒复制依赖 活化NF—KB信号通路 林珊珊1邬敏1，2徐杨1熊炜1张小楠1，2易志刚1袁正宏1，2，3 【摘要】 变体，获得核因子kappa core蛋白的合成显著降低；而 质粒共同瞬转细胞后，转染MyD88和HBV表达质粒的细胞中NF_KB的通路明显活化，同时HBV 【关键词】 B 乙型肝炎病毒；髓样细胞分化蛋白；抗病毒活性；核因子kappa Inhibitionof Bvirus ismediatednuclear Bactivation hepatitisreplicationbyMyD88 by factor-kappa MedwdMo如cular 删Shah-shah，WUMin，删Yang，Xiong耽i，旎僦Xiao-nan，YI of Virology，．站硼砌缸MedicalCollege，FudanUniversity，Shanghai200032) 【Abstract】 To themechanismof inhibitionof B Two Objectiveinvestigate MyD88 hepatitis tnmcatedversionsof constructed．HasmidIrBa-SR NF-xB or MyD88，M(1-151)andM(152．296)were encodingsuper-repressorplasmid IKK矗／ⅨKBwhichcall activateN】nKB wereused．Huh7cellswere transfectedwithHBV ortIle strongly signaling transiently dimer．MyD88 above of DNAandNF-tcBactivationwere The 0ft11e plasmids．AssaysnBsAg，HBeAg，coreprotein，HBV performed．Resultsexpression M1 thetwotruncated differentinductionofNF-tcB lengthMyD88，and forms，M(1-151)andM(152—296)，showed patterns aetivi押；patterns thatareconsistentwiththeir effectonviral andcore HBVDNA inhibitory proteinsynthesis particle-associated of resultedina reductionofcore andthe inhibitedthecore expression significant co-expressionofⅨ‰／IKIK8 MyD88 protein dramatically level．Furthermore．theof restoredthecore level．Similar also p