细胞生物学测试题.docVIP

一、填空题 　　1 . Cyclic AMP is produced by the enzyme　　　　and degraded by the enzyme　　　　。 　　2 . NO是一种气体信号分子,在体内主要由精氨酸产生, 它的半衰期很短, 故只作用于邻近细胞, 它作用的靶酶是　　　　。 　　3 . 　　　　junctions enable cells to get a hold on the extracellular matrix by connecting their actin filaments to the matrix. 　　4 . 动物细胞质膜靠 建立膜两侧的质子动力势，而植物细胞则是靠　　　　。 　　5. 蛋白质合成时多聚核糖体的形成对生命活动的意义在于:　　　　。 　　二、判断题 　　1. Active transport is always driven by the hydrolysis of high-energy phosphate bonds.（　　　） 　　2. Treatment of an animal cell with an inhibitor that is specific for the Na+/K+ pump is not likely to affect the uptake of glucose by sodium cotransport.（　　　） 　　3. Tight junctions get their name from their property of holding cells togetherso tightly that they cannot be separated by mechanical forces.（ ） 　　4. Gap junctions connect the cytoskeletal elements of one cell to a neighboring cell or to the extracellular matrix.（ ） 　　5. NO acts only locally because it has a short half-life--about 5 to 10 seconds--in the extracellular space before it is converted to nitrates and nitrites by oxygen and water.（　　　） 　　三、选择题 　　1. Which one of the following was used to determine the structure of the DNA molecule?（　　　） 　　a. Transmission electron microscope b. Scanning electron microscope 　　c. Differential centrifugatioin d. X-ray crystallography 　　e. None of the above 　　2. You stain membrane proteins with a fluorescent-labeled antibody and notice the membrane is evenly stained. After a few hours, all of the fluorescence is at one end of a cell. You can conclude that（　　　）. 　　a. Lipids flip-flop in the membrane. b. Proteins move laterally in the membrane. 　　c. The fluorescent dye was bleached. d. Proteins act as transporters. 　　e. All of the above 　　3. You have a preparation of ghosts and need to collect only the right-side out ghosts. You decide you can trap the right-side out ghosts in an affinity column packed with（　　　） 　　a. SDS. b. A carbohydrate-binding molecule. 　　c. A protein-binding antibody. d. A serine-binding molecule. 　　e.