柏树火焰层孔菌菌丝体的液体培养-林业科学研究.PDF

林 业 科 学 研 究 2010, 23( 1) : 98 ～ 10 1 Forest Research 文章编号: 1001- 1498( 2010) 0 1-0098-04 柏 树 火 焰 层 孔 菌 菌 丝 体 的液 体 培 养 1 1* 2 苏 珏 , 贺新生 , 郑林用 ( 1. 西南科 技大 学生命 科学 与工程 学院, 四川 绵阳 621010; 2. 四川农 业科 学院, 四川 成都 6 10000) 摘要: 柏树火焰层孔菌是柏树的病原菌物, 是中国和亚洲的新记录属 中的新记录种。本文采用液体发酵的方法对菌 丝体生长的营养条件等生物学特性进行了研究。结果表 明, 柏树火焰层孔菌菌丝体生长最适碳源为淀粉, 最适氮源 为麸皮, P、K、Mg 对其菌丝的生长具有促进作用, Fe、Zn 对其 菌丝生长具有显著抑 制作用 。通 过生长 曲线试验得到 Logistic 方程: y = 0. 22 / ( 1 + 1. 0 19 e - 0. 013x ) , 菌丝体干质量为 0. 22 g ·100 mL- 1 。 关键词: 柏树火焰层孔菌; 液体培养; 菌丝体产量 中图分类号: S7 18. 8 1 文献标识码: A The Liquid Cultivation of the Mycelia of Pyrofomes demidoffii 1 1 2 SU Jue , HE Xin-sheng , ZHENG Lin-yong ( 1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China; 2. Sichuan Academy of Agricultural Science, Chengdu 610000, Sichuan, China) Abstract: Pyrofomes demidoffii is a new recorded species of the new recorded genus in the China and Asia, and a pathogenic fungus of j uniper. Adopting the liquid fermentation to study its biological characteristics such as vegetal nutrition condtion of mycelia, the results indicated that the mycelia of P. demidoffii could be produced by liquid fermentation process. Starch and bran were the optimum carbon and nitrogen source, and P, K, Mg had the effection of acceleration to the growth of mycelia, Fe, Zn inhibited its growth. The growth curve of the logistic equation was deduced: y = 0. 22 / ( 1 + 1. 0 19e - 0. 0 13x ) , the weight of the dry mycelia was 0. 22 g ·100 mL - 1 . Key words: Pyrofom