(中斡肽)哺乳动物基因组转录诱导融合基因的研究进展.doc

投稿函 （1）南阳理工学院计算机科学与技术系，南阳 473004 (2）中国科学院北京基因组研究所，北京 101300; 摘要Q7 * 国家自然科学基金重点项目和教育部重点项目（02090）资助项目（注明资助项目类别、名称和批准号） **通讯联系人：吕聪颖 Tel：0377 E-mail：Alin0378@ 收稿日期：2007-4-9 Progress of the transcription-induced fusion gene in eukaryotic genomes* Lv CY1), Zhao GB1,Lv GT12) (1) College of Computer Science and Technology, Nanyang Institute of Technology, 473004; (2)(Beijing Institute of Genomics, Chinese Academy of Science, Beijing, 101300 Abstract Eukaryotic genomes are composed of individual genes and the intergenic regions. The transcription of a gene always begins from the transcription start site and ends at the termination site; the gene is referred as an individual transcription unit. However, some sporadic studies indicate that transcription can sometimes read-through the intergenic region and generates a large fusion transcript which contains the upstream gene, intergenic region and the downstream adjacent gene. The fusion transcript becomes a mature functional transcript after intergenic splicing. The article summarizes patterns of intergenic splicing, possible generation mechanism and its significance. Key words gene，transcription, fusion gene * This work was supported by a grant from the National Natural Science Foundation of China (Grant No., the Key Project of Chinese Ministry of Education (Grant No. 02090). **Corresponding author. Tel: 0377 E-mail:Alin0378@ Received: 2007-4-9 Accepted: 在真核生物基因组中，基因（特指蛋白编码基因）被不编码的基因间区（intergenic region）所隔开，形成独立的转录单元[1,2]. 基因在转录时，从特定的起始位点（transcription start site, TSS）开始，把编码区转录出来后，在该基因的终止位点（termination site）结束[2,3]. 然而，近年来有零星的报道表明[4-8]，在转录过程中，RNA聚合酶II（Pol II）有时会通读（read through）终止位点和基因间区，在下游基因的转录终止位点结束，产生一个含有上游基因、基因间区和下游基因的融合转录本. 剪接体（spliceosome）对其进行剪接时，把基因间区及其附近的位于上下游基因的UTR（untranslated region）甚至部分外显子作为一个大的内含子从转录本中切除，从而形成有功能的融合转录本（图1），该转录本有时还含有来自于基因间区的新外显子. 为了和单个基因的剪接有所区别，这种发生在相邻基因间的剪接被称为基因间剪接（intergenic splicing）[4]. 我们称这种由于基因的转录而产生融合基因的现象为转录诱导的融合基因（transcripti