DNA测序技术的发展历史及最新进展.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 2010 8 DNA 解增言 林俊华 谭军 舒坤贤 (, 400065) : DNA 测序技术是现代分子生物学研究中最常用的技术从 1977 第一代测序技术的出现, 经过 30多 的发 展, DNA 测序技术取得重大进展,以高通量为特点的第二代测序技术逐步成熟并商业化, 以单分子测序为特点的第三代测序 技术也已经出现介绍每 一代测序技术的特点,并重点介绍了第二代测序技术及其应用展望新的测序技术对于未来生物 学研究及人们自身健康与人类疾病等方面研究的影响 : 第一代 DNA测序技术 第二代 DNA测序技术 第三代 DNA 测序技术 高通量 单分子测序 The H istory and Advances of DNA Sequencing T echnology X ie Zengyan Lin Junhua Tan Jun Shu unxian (Co lleg e of B ioinf orm a tion, Chong qing Un iversity of P osts and T elecommunica tions, Ch ong qing 400065) Abstract: As the commonly used technology in molecular biology, DNA sequencing technology has seen great advances in more than thirty years since 1977. NextgenerationDNA sequencing technology, characterized by highthroughput, has entered themarket and been growing to maturity. The thirdgeneration sequencing technology has also arisen, which can sequence single DNA moleculars. In this review, we introduced three generations of sequencing technologies and emphasized on the secondgeneration sequencing technolo gy and its applications. At the end of the review, the effect of new DNA sequencing technologies on research and medicinewere pre viewed. Key w ords: F irstgeneration DNA sequencing technology Secondgeneration DNA sequencing technology Thirdgeneration DNA sequencing technology H ighthroughput Singlemolecular sequencing technology. DNA , 1 DNA DNA 20 701977 DNA , RNA [ 1] Maxam Gilbert 1949 , Frederick San [2] [3] DNA, Sanger ger 20 90 , 1953 DNA