新型肝素化聚氨酯膜的制备及研究_王珺斌.pdf

DOI :0.4 35/ki .006 -3080.2008.05.0 ( ) Vol.34 No.5 704 　　　　　　Journal of East China University of Science and Technology (Natural Science Edition) 2008-0 　　:006-3080 (2008)05-0704-05 王 斌, 　赵燕超, 　龚飞荣, 　饶　炬, 　程树军 (华东理工大学材料科学与工程学院,超细材料制备与应用教育 重点实验室, 上海200237) 　　:通过两步溶液聚合法合成表面含有羧基的聚氨酯(PU), 并用 -乙基-3-(二甲基丙胺)碳 二亚胺(WSC)对聚氨酯表面羧基进行活化后, 在4 ℃下柠檬酸钠缓冲液中与肝素上氨基反应得到 表面共价键接枝的肝素化聚氨酯膜。通过甲苯胺蓝染色法、红外光谱法等对肝素化前后结构特征 进行表征, 用静态水接触角、体外抗凝血性测试其生物相容性。结果表明:在pH=5.0 的肝素柠檬 2 酸钠缓冲液中反应96 h,聚氨酯表面肝素接枝率最高,接枝量达到 .92 μg/cm ;同时, 共价接枝肝 素后接触角从6 °降低到44°,并且抗凝血性能得到明显的改善。 :聚氨酯;羧基;肝素;共价键;抗凝血 :TQ32;R3 8 :A A Novel Polyurethane Immobilized with Heparin WANGJ un-bin, 　ZHAO Yan-chao, 　GONGFei-rong, 　RAOJ u, 　CHENGShu-j un (K ey Laboratory f or ltraf ine Materials of Ministry of Education, Schoolof Materials Science and Engineering,East China niversity of Science and Technology, Shanghai 200237, China ) Abstract:Polyurethanes containing carboxyl group w ere synthesized by tw o-step solution polymeri- zation.Heparin w as immobilized on the PU surface via reacting betw een carboxyl group and the amino group in the heparin by covalent immobilization technology in heparin sodium citrate buffer at 4 ℃.The heparinized polyurethanes were investigated by FT-IR, w ater contact angle and antithrombin tests.Results show that the highest heparin content of PU surface is in heparin sodium citrate buffer (pH=5.0)for 96 h. The w ater connect angle is reduced from 6 °to 44°after heparinization.Meanw hile, heparinization observably improves the blood anticoagulation of polyurethane. Key words:polyurethane;carboxyl group;heparin;covalent bond;blood anticoagulation 　　(PU)、、 。, [ ] 。 [2]