水稻葡萄糖_6_磷酸脱氢酶cDNA电子克隆_黄骥.pdfVIP

, 29(11):1012 ～ 1016, 2002 Acta Genetica Sinica -6-cDNA 1 1 1, ① 1 2 1 2 黄　骥 , 王建飞, 张红生 , 曹雅君,林长发, 王　东,杨金水 (1., 210095;2., 200433) :。-6- cDNA (Tagpdl ), GenBank nr , RT-PCR cDNA , OsG6PDH。OsG6PDH Tagpdl DNA 88%, 、、-6- 89%、79%、80%。 RT-PCR , OsG6PDH 、、、, 。, 。 :;;-6-;RT-PCR :Q75　　:A　　:0379-4172(2002)11-1012-05 In Silico Cloning of Glucose-6-Phosphate Dehydrogenase cDNA from Rice (Oryza sativa L.) 1 1 1, ① 1 2 H ANG Ji , WANG Jian-Fei , ZHANG Hong-Sheng , CAO Ya-Jun , LIN Chang-Fa , WANG 1 2 Dong , YANG Jin-Shui (1.NationalKey Laboratory of Crop Genetics and Germplasm Enhancement, Nanj ing Agric lt ral University, Nanjing 210095, China;2.Instit te of Genetics, F dan University , Shanghai 200433, China) Abstract:In silico cloningwas anew strategy of gene cloning developedwith the development of genome, EST projects and bioinformatics.singwheat glucose-6-phosphate dehydrogenase cDNA (clone:Tagpdl)sequence as a querying probe, one highly homologous BAC clone sequencewas obtained from rice sequence database of GenBank and the putative cDNA sequence of riceglucose-6-phosphate dehydrogenasewas assembled according to thewheat clone.Futhermore, the full-length cDNA of rice glucose-6-phosphate dehydrogenase was cloned by RT-PCR with two primers designed based on this assembled cDNA sequence.Sincethis fragment contained a complete ORFof 1515bp with a stop codon in its upstream and poly(A)signal in its downsteam, it could be concluded that a full-length gene (GenBank accession number AY078072), which was named