多菌灵在黄精根茎无菌培养中的应用-生物技术通报.PDF

生物技术通报 BIOTECHN OLOG Y B ULLETIN 2006 1, 2 3 1 2 2 徐忠传 何俊蓉 周静亚 徐晖 徐凯 1 2 ( , 215500; 3 , 310023; , 610066) : 本研究中比较了几种杀菌剂对黄精根茎的灭菌效 结 表明, 采用乙醇饱和漂白粉HgCI 等杀 2 菌剂对黄精根茎杀菌时,微生物的污染率很高; 先用 3% 多菌灵溶液对黄精根茎进行内吸杀菌处理 45h以上, 然后 再用 02% HgCI 进行灭菌 10m in, 则黄精根茎的污染率可得到有效控制多菌灵的处理效 与黄精根茎的大小 2 类型和年龄等因素有关 : 多菌灵 多花黄精 污染率 组织培养 Application of Carbendazim in Sterile Culture of Polygonatum Cyrtonema H ua R oots 1, 2 3 1 2 2 Xu Zhongchuan H e Junrong Zhou Jingya XuHui Xu ai 1 ( Dep tartment of B iology Science and Food Engineering, Changshu college of Science and Technology, 2 Changshu, Jiangsu 215500; Dep tartment of B iolog ical and Chemical Engineering, Zhejiang niversity of 3 Science and Technology,H angzhou 310023; lnstitute of B iolog ical andN uclear Technology, SichuanA cademy of Agricultural Science, Chengdu 610066) Abst ract: The effect of some kindsofbactericides have compared in this paper. The results showed thatm icrobe pol- lution ratewas very high, ifPolygonatum cyrtonema Hua rootswere dealed with ethano,l saturated bleaching power orHg- CI. Polygonatum cyrtonema Hua roots pollution ratewere controlled b