天津主要河流中耐高温蛭弧菌的分离及生物学特性-食品与生物技术学报.PDF

第30 卷第2 期 食 品 与生 物 技 术学 报 Vol. 30 No. 2 2011 年3 月 Journal of Food Science and Biotechnology M ar. 2011 :2011) 天津主要河流中耐高温蛭弧菌的 分离及生物学特性 黄 亮, 宋虹霖, 王 莉, 丁辉, 马 呈, 童应凯* ( 天津农学院农学系, 天津300384) : 从天津4 条主要河流( 流减河, 南运河, 北运河, 子牙河) 中分离出了耐高温的蛭弧菌并 进行了纯化通过采用宿主双层琼脂平板法对从河水中筛选出耐高温菌株并对其进行宿主范围 pH温度等生物学特性的测定, 结果表明: 耐高温蛭弧菌在pH 70~ 75 生长最好; 对热致死的大 肠杆菌, 嗜水气单胞菌, 荧光假单胞菌等3 种细菌仍有裂解作用且出斑时间较裂解活菌早; 寄生谱 测定发现其可侵染绝大多数革兰氏阴性菌和部分革兰氏阳性菌, 且为专性寄生 : 蛭弧菌; 耐高温菌株; 生物学特性 : Q 939. 97 : A Purification of Bd ellovibrio with High Temperature Resistance in Tianjin Rivers and Preliminarily Studies on Its Biological Characteristics * HUAN G Liang, SONG Honglin, WANG Li, DING Hui, MA Cheng, T ONG Ying ai ( Department of Agricult ure, Tianjin Agricultural University, Tianjin 300384, China) bstract: Bde ll ov i br i o w hich could grow n at high temperature conditions w as screened and purified in T ianjin four main rivers. T he biological characteristics w ere tested by double dec agar plate, including its host spectra, pH resistance, and cultivation temperature. T he results show ed that the Bd ell ov i br i o with high temperature resistance grow ed w ell at pH 70~ 75, and it could prey on the dead bacteria earlier compared w ith the living bacteria, such as Escher ic hi a c ol i, A er omonas hy dr op h i la, P seudomonas f l uor escens , mass of gram negative bacteria and some gram positive bacteria could be preied on by the Bdellovibrio, w hich is an obligate parasite. Key words: Bde ll ov i br io; thermostable strain; biological characteristics ( Bd