pBacPAK8昆虫表达载体说明.pdf

pBacPAK8 编号 载体名称 北京华越洋生物KCVECT129 pBacPAK8 pBacPAK8 载体基本信息 载体名称: pBacPAK8 质粒类型: 昆虫表达载体；杆状病毒表达载体 克隆方法: 限制酶，多克隆位点 启动子: Polyhedrin 载体大小: 5538 bp 5 测序引物及序列: Bac1 Forward 3 测序引物及序列: Bac2 Reverse 载体标签: 无标签 载体抗性: 氨苄青霉素 真核筛选标记: 无 克隆菌株: TOP10 宿主细胞（系）: 果蝇细胞系IPLB-Sf21 备注: -- 瞬表达/稳表达: 稳表达 组成型/诱导型: 组成型 病毒/非病毒: 杆状病毒 pBacPAK8 载体质粒图谱和多克隆位点信息 pBacPAK8 载体描述: Available as part of the BacPAK Baculovirus Expression System (Cat. No. 631402). pBacPAK8 is a transfer vector designed for high-level expression of a cloned gene driven by the strong AcMNPV polyhedrin promoter. Flanking AcMNPV sequences allow recombination with viral DNA to transfer the expression cassette to the polyhedrin locus of the viral DNA. The polyhedrin coding sequences have been replaced by a multiple cloning site with 18 unique sites that facilitate the insertion of foreign genes in the correct orientation for expression. The Pac I site at the end of the MCS region provides translational stop codons in all three reading frames for expression of truncated proteins. pBacPAK8 has a pUC origi