pCoBlast昆虫表达载体说明.pdf

pCoBlast 编号 载体名称 北京华越洋生物KCVECT105 pCoBlast pCoBlast 载体基本信息 载体名称: pCoBlast 质粒类型: 昆虫表达载体；筛选载体 克隆方法: -- 启动子: copia 载体大小: 3907 bp 5 测序引物及序列: -- 3 测序引物及序列: -- 载体标签: 无 载体抗性: 氨苄青霉素 真核筛选标记: Blasticidin 克隆菌株: TOP10 ；DH5-T1R 宿主细胞（系）: 果蝇细胞系S2 备注: pCoBlast 载体与昆虫表达载体共转染，建立稳表达细胞系。 瞬表达/稳表达: -- 组成型/诱导型: 组成型 病毒/非病毒: 非病毒 pCoBlast 载体质粒图谱和多克隆位点信息 pCoBlast 载体简介 pCoBlast is a 3907 bp selection vector that can be cotransfected with the expression vector of choice to to create stable cell lines in Drosophila. It contains the Streptomyces griseochromogenes bsd gene under control of the Drosophila copia promoter to confer resistance to blasticidin in S2 cells. 引用及参考文献 A novel allelic variant of the human TSG-6 gene encoding an amino acid difference in the CUB module. Chromosomal localization, frequency analysis, modeling, and expression. Nentwich Hilke A; Mustafa Zehra; Rugg Marilyn S; Marsden Brian D; Cordell Martin R; Mahoney David J; Jenkins Suzanne C; Dowling Barbara; Fries Erik; Milner Caroline M; Loughlin John; Day Anthony J; J Biol Chem (2002) 277:15354-15416 Product usage: The full-length coding sequence of human TSG-6 (A431 allele encoding Gln at amino acid 144), including the signal sequence and stop codon, was excised from the pAcCL29-1 vector by digestion with KpnI/XbaI and cloned into the corresponding sites in the Drosophila Expression System pCoBlast 载体序列 TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCT GTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCT