解淀粉芽孢杆菌Ba33对烟草的促生及抗性.pdfVIP

2010, 32(4) :383~ 386 http : xuebao.j lau . edu . cn J ournal of J ilin Agricultural University E2mail :j lndxb@vip . sina . com Ba33 TMV X 申莉莉, 王凤龙, 钱玉梅, 杨金广 , 266101 : 从烟田土壤中分离得到解淀粉芽孢杆菌( Bacillus amyloliquef aciens ) Ba33 菌株5 叶期 生NN 烟 ( Nicotiana ta bacum var. samsun NN) 喷施Ba33 发酵液3 次后再接种TMV( 烟草花叶病毒) , 与喷清水对照相比, 能 显著降低枯斑数量, 枯斑抑制率为671 8% 于4~ 6 叶期普通烟草( Nicotia na tobacum L.) NC89 烟苗接种TMV 前后分别用 Ba33 发酵液NB培养基或清水连续灌根3 次, 观察到Ba33 发酵液灌根处理的烟苗鲜重最大叶 长和叶宽均高于清水对照处理, 叶色浓绿, 长势良好; 接种TMV 10 d 后进行实时荧光定量PCR 检测,Ba33 发酵 液灌根处理的植株新叶中RNA 含量低于清水对照和NB 培养基灌根处理认为Ba33 具有拮抗TMV促进烟 草生长的作用,是具有潜在应用价值的生防菌株 : 解淀粉芽孢杆菌; 烟草; 烟草花叶病毒; 抗病性 : 435172 : A : 10002 5684( 2010)042 03832 04 Tobacco Gr owth2Pr omotion Effect an d TMV Resistance of Bacillus amy2 loliquef aciens Ba33 SHEN Li2li, WANG Fe ng2long, QIAN Yu2mei, YANG J in2gua ng Tobacco Research Institute, China Academy of Agricultural Sciences, Qingdao 266101, China Abstract: A Bacillus amyloliquefaciens named Ba33was isolated fromtobaccofields. Nicotiana tabacum var. samsum NN seedlings with 5 leaves sprayed with Ba33 ferment liquid 3 times and then inoculated with TMV showed lesser number of necrotic lesions compared with control, and its suppression ratio of necrotic lesions was 6718%. Before and after inoculatingTMV, seedlingswith 4) 6 leaves of Nicotiana tobacum var. NC89were irrigated with Ba33 ferment fluid, NB and water 3 times. The results showed that fresh weight, big leaf length and width of Ba33 treatment were better than water treatment, showing leaf green and good growth. The RNA content in new leaves of Ba33 treatment was lower than the tobacco of NB and water treatment by the real time quantitative PCR. Ba33 was a potential bio2control bacterium with anti2virus and gro