促红细胞生成素对坏死性小肠结肠炎TLR4NFB与IL6变化的影响.pdf

91 2011 9 1 36 9 Med J Chin PLA, Vol 36, No 9, Septem ber 1, 2011 促红细胞生成素对坏死性小肠结肠炎TLR4NF- B 及IL-6 变化的影响 陈晓晴, 许华, 罗小平 [ ] (EPO) ( NEC) TLR4NF-B IL-6 , EPO NEC 90 3 SD 3 ( 30 ) : NEC EPO NEC EPO EPO(01U/ ml) , , 244 72h 10 3cm, H E , 2 NEC PCR ELISA TLR4 mRNANF-B mRNA IL-6 NEC TLR4 mRNA 24h , 4 h 72h , (P 001) EPO 24h , , 4 h 72h NEC (P 001) NEC NF-B mRNAIL-6 4 h 72h (P 001) ; EPO 4 h , 72h NEC (P 001) 4 h , 72h NEC EPO , NEC (2 3 056) EPO ( 153 035,P 001) NEC , EPO TLR4 , , [ ] , ; , ; ; Toll 4 [ ] R725745 [ ] A [ ] 0577-7402(2011)09-091-05 Effect of erythropoietin on dynamic changes of TLR4, NF-B, and IL-6 in rat model with necrotizing enterocolitis * CHEN Xiao-qing, XU Hua, LUO Xiao-ping Department of Pediatrics, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China * Corresponding author, E-mail: xpluo@tjh. tjmu.edu. cn Abstract] Objective To explore the protective effect and the mechanism of erythropoietin ( EPO) on dynamic expressions of TLR- 4, NF-B, and IL-6 in the intestinal tissue of neonatal rat model with necrotizing enterocolitis ( NEC) . Methods A total of 90 SD rats ( 3 days old) were divided randomly into 3 groups ( 30 each) as follows: normal control group, NEC group, and EPO group. The NEC rat model was established by feeding the subjects with rat-milk substitutes after separation from the mother rats. The subjects were then exposed to hypoxia and cold stress. The EPO-treated rats were fed with the substitute of rat-milk containing EPO (01U/ ml) , and were exposed to hypoxia and cold stress as well. The rats in the control group were raised by their mothers and received no intervention. Ten rats from each group were sacrificed at 24, 4 , and 72h, respectively. The last 3cm of the terminal ileum was excised and t