同源四倍体水稻恢复系TP-4及D明恢63及保持系D46B的细胞遗传学比较.pdfVIP

HEREDITAS (Beijing) 2007 4 , 29(4): 462470 ISSN 0253-9772 DOI: 10.1360/yc-007-0462 TP-4 D 63 D46B , , , , , , , 610041 : TP-4 D 63 D46B 2n = 48, TP-4 D 63 D46B , 99%, TP-4 D 63 2.00/PMC 2.26/PMC, D46B 6.00/PMC, ; D46B 10.62%, TP-4 19.44%D 63 23.14%, 63 7.30%; D46B D46B , : ; ; ; ; u ; Cytogenetical comparison of restorers TP-4 and D minghui63 and maintainer D46B of autotetraploid rice LONG Wen-Bo, LUAN Li, WANG Xing, LIU Yu-Hua,TU Sheng-Bin, KONG Fan-Lun, HE Tao Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China Abstract: Cytogenetical comparison was made between high seed set restorers TP-4 and D minghui63 and eminent main- tainer line D46B of autotetraploid rice. The meiosis observation demonstrated the genomes of our autotetraploid materials were all 2n = 48, the same as those in mitosis observation. Low percentages of univalent and trivalent in metaphase I (MI) of restorers TP-4 and D minghui63 and in metaphase I (MI) of maintainer line D46B of autotetraploid rice were observed. And the percentages of chromosome pairing were all over 99%, showing eminent cytological character. The frequency of TP-4 and D minghui63 in metaphase I (MI) was 2.00/PMC and 2.26/PMC, respectively. However the frequency of D46B was 6.00/PMC, significantly higher than those of TP-4 and D minghui63. It indicated that the maintainer D46B has better chromosome pairing capability in metaphase I (MI). While, the frequency of lagging chromosomes of the maintainer D46B in anaphase I (AI)