大豆7S球蛋白alpha-亚基缺失型种质创新幻灯片.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2010, 36(8): 14091413 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.01409 大豆7S 球蛋白α-亚基缺失型种质创新 刘珊珊 滕卫丽** 姜自芹 张彬彬 葛玉君 刁桂珠 郑天慧 曾 蕊 吴 帅 李文滨* / , 150030 : , , 10 () (α+α+11S )- B F1 , F2 (SDS), F1 7S α-α- F α-(α+A A A )- A -(α+A )- 2 1a 1b 2 3 4 (α+α)- , : ; 7S ; α-; Development of Soybean Germplasm Lacking of 7S Globulin α-Subunit ** LIU Shan-Shan, TENG Wei-Li , JIANG Zi-Qin, ZHANG Bin-Bin, GE Yu-Jun, DIAO Gui-Zhu, * ZHENG Tian-Hui, JIANG Zi-Qin, ZENG Rui, WU Shuai, and LI Wen-Bin Soybean Research Institute, Northeast Agricultural University / Key Laboratory of Soybean Biology, Ministry of Education, Harbin 150030, China Abstract: α-subunit of soybean β-conglycinin is one of the major allergens in soybean products. It is possible to reduce or remove the allergens from soybean by development of mutant lines. In this study, a cross was made between 10 major varieties in Heilongji- ang province of China and “riB” lacking α-, α-, and 11S acidic subunits in order to breed soybean variety characterized by α-subunit null. F seeds were sown individually and individual plants were harvested to obtain F -progeny seeds. Subunit compositions of seeds 1 2 in F and F were examined for storage protein by SDS. The results showed that all the F seeds obtained from crosses con- 1 2 1 tained α- and α-subunits. F