辣椒素对离体豚鼠乳头状肌电生理效应.pdfVIP

ActaPhysiologicaSinica,October25.200355(5):511一515 511 Electrophysiologicaleffectsofcapsaicinonguineapigpapillary musdes LfQian,CHENGYan-Ping,HERui-Rang DepartmentofPhysiology，!mutateofBasicMedicine,HebeiMedical Slujiazhuaag050017 Abstract; Thecardiacelectrophysiologicaleffectsofcapsaicin(CAP)wereexaminedinguineapigpapillarymusclesusing intracellularmicroelectrodetechnique.Theresultsobtainedareasfollows:(1)thedurationofactionpotential(APD)innor- matpapillarymuscleswasdecreased场CAP(30,60,120pmol/L)inaconcentration-dependentmanner;(2)inpartially depolarizedpapillarymuscles,60lmal/LCAPnotonlyreducedAPD,butalsodecreasedtheamplitudeofactionpotential, overshootandmaximalvelocityofphase0depolarization;(3)pretreatmentwithL-typeCa，十channelagonistBayK8644(0.5 pmcl/L)couldcompletelyblocktheeffectsofCAP(60Vmol/L);(4)pretreatmentwithrutheniumred(20pmol/L)，a vanilloidreceptor(VR)blacker,didnotaffecttheactionsofcapsaicinonpapillarymuscles.Alltheseresultssuggestthatthe effectsofCAPonpapillarymusclesarelikelyduetoadecreaseincalciuminfluxwhichisnotmediated切VR. Keywords:physiology;capsaicin;actionpotential;papillarymuscles 辣椒素对离体豚鼠乳头状肌的电生理效应 李 茜，程岩萍，何瑞荣 ‘ 河北医科大学基础医学研究所生理室，石家庄050017 摘 要: 应用细胞内微电极技术观察了辣椒素(capsaicin,CAP)对豚鼠乳头状肌细胞的电生理效应。结果表明: (1)CAP(30,60,120l,,moUL)可浓度依赖地缩短正常乳头状肌的动作电位时程。(2)对部分去极化乳头状肌，CAP (60lemol/L)除缩短动作电位时程外，还使动作电位幅值和超射值降低，零相最大上升速度减慢。(3)预先应用L型 钙通道开放剂BayK8644(0.5ltmoVL)，则可阻断CAP(60pmal/L)的电生理效应。(4)预先应用辣椒素受体(va- nilloidmceptor,VR)阻断剂钉红((20h,tnol/L)，不影响CAP(60p.mol/L)的电生理效应。以上结果提示，CAP可能通过 非受体途径抑制Ca‘内流，从而影响豚鼠乳头状肌电生理效应。 关键词:生理学;辣椒素;动作电位;乳头状肌 中图分类号 Q463 Capsaicin(CAP)，themainpungentcomponentof from thevanilloid-sens