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Tissue-Specific Stem Cells
Cartilage-Derived Stromal Cells: Is It a Novel Cell Resource for
Cell Therapy to Regenerate Infarcted Myocardium?
WENJUN SU,a HAO ZHANG,a ZHUQING JIA,b CHUNYAN ZHOU,b YINGJIE WEI,a SHENGSHOU HUa
aResearch Center for Cardiovascular Regenerative Medicine, the Ministry of Health, and Department of
Cardiovascular Surgery, Cardiovascular Institute and Fu-Wai Hospital, CAMS and PUMC; bStem Cell Center,
Beijing University, Beijing, People’s Republic of China
Key Words. Myocardial infarction • Cartilage • Stromal cells • Cell transplantation
ABSTRACT
Human cartilage is reported to contain multipotent stro- plantation. Echocardiography indicated that CDSC
mal cells. We evaluated the effect of human cartilage- transplantation could improve heart function. The num-
derived stromal cells (CDSCs) on heart function when ber of capillaries increased in the injection regions in the
transplanted into the infarcted myocardium of rats. transplantation group. Histological examination showed
CDSCs were isolated and cultured from human articular that Hoechst-labeled CDSCs in islands within the in-
cartilage and subjected to fluorescence-activated cell sort- farcted region were stained positively for desmin and
ing (FACS) analysis. The CDSCs were consistently nega- smooth muscle actin but negatively for alpha-sarcomeric
tive for CD14, CD34, CD38, CD45, CD49f, CD104, actin and troponin-I. RT-PCR results indicated the ex-
CD105, CD106, CD117, HLA-DR, and ABCG-2, and pos- pression level of collagen I, collagen III, tissue inhibitor of
itive for CD10, CD44, CD71, CD73, CD90, CD147, and met
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