鉴定猪,牛和绵羊肥大细胞的组织化学技术(英文).pdfVIP

维普资讯 ， I＼7卵 () 动 物 学 研 究 1998，19 (I)：l—l0 CN 53--1040／Q ISSN 0q,54-5853 ZoologicIlResearch HISTOCHEMICALTECHNIQUESIDENTIFYING M AST CELLS IN PIG，CATTLE AND SHEEP xu ke——ren JIANG Ping (DepartmentofAnimatScience，GuizhouAgriculturalCollege．G iyang, 550025) Abstract Carnoysfluidand neutralbufreredofrmalin (NBF) havebeellproved tobe goodfixativesforpreservationofmastcellsinpig，cattleandsheepexceptNBFblocked stain- ingofmostporcinemastcells，especiallythoselpeated in lntesfina1mucoski(MM C)and iu thymusmedulla(TMMC)BothtotuidincblueandAktanbluewere theexcelleatstamsgener． ally，butAIcianbluestainedmoreporcinemastcellsthandidtoluidineblue (，0．0l1．Stain- ingwithtoluidineblueofawidepH range (from 0．1t070)showedthat∞ nomastcells wercnotverypH dependent，butthedyeatpH 0，5seemed tohavethestrongestaffinityofrall mastcellsin pigsand itwasalso suitableforbovineandovinemastce llstaining ． In thethree species，unlike1nrodents，theAlctanbluemethoddidnotdistinguishbetweenmastcellsinthe intestina1mucosa (MM C) and those 1n theconnectivetsisueofthe intestinalsubmucosa． tongueandskin (CTMC)．PorcineCTMC，butnotMMC，fluorescedstronglywhenstained withbetbefinesklipbateorwith amixtureofberbefinesulphateandacridineorange．Itsug． gestedthatporcineCTM C containedhcparJnprotcoglyCal1 Keywords M astcell，Histochemstry，Pig，Cattle，Sheep M astcellswere originally identified in ratconnective tissue by the numerous basophilicandmetachromaticstainingpropertiesoftheirprominentcytoplasmicgranules (Ehrlich， l879)anditisknownthattoluidineblue．Alcianblueandberberinesulphate s