MALDI Biotyper高通量微生物鉴定系统在台湾进口鳖蛋检疫中的初步应用.pdfVIP

JOURNA L OF IN SPECT ION AN D QU ARANT INE V ol 2 1 N o 1 20 11 1 MALD I B iotyper 徐淑菲 孔繁德 ( 3610 12) [ 目的] 应用MALD I- TOF - M S 和 B io typ er分析软件对台湾进口的鳖蛋进行携带病原菌的检测 [ 方 ] 按照病原菌检测标准进行培养, 选取可疑菌株, 肉汤培养后, 按照MALD I- TOF - MS 要求进行样品制 备点靶以及样品质谱图的获取, 并运用B iotyper分析软件对所获取的质谱图进行分析[结论]应用MA LD I- TOF - MS 方 从进口鳖蛋中分离出绿脓杆菌奇异变形杆菌肺炎克雷伯氏菌等致病菌, 为进出口商检验机构 及其他相关部门敲响警钟此鉴定方 简单快速, 能够加快鳖蛋的通关速度, 为两岸建设作贡献 鳖蛋; MA LD I- TOF - M S; 质谱图; 病原菌 O 657 63; Q 939 The TrialApplication ofMALDI BiotyperAppraisal System in the Inspection of theTerrapin Eggs from Taiwan X u Shufe,i K ong F ande ( X iam en Entry- Ex it In sp ection and Qu arantine Bureau, X iam en, Fu jian, 36 1012) Abstract: [ Ob jectives] T o app ly the MA LD I- TOF - M S and B io typ er analysis sofwt are in invest igating the p atho genic b acteria con tam ination in terrap in eggs from T awi an [ M ethods] To cu lture the b acter ia according to the standard m ethod, after culturing w ith broth, the prob ab le p athogen ic germ s are prep ared and added to the target po lished stee l plate and the m ass sp ectrum draw ings are acqu ired T he draw ings w ere analysised by the B iotyp er a nalysis softw are [ C onclu sion s] The p athogen ic germ s such as Pseudom onas aerug inosa, P ro teu s m irab ilis, K leb siella pneum oniae, etc w ere separated from the terrap in eggs from T aiw an by MALD I- TOF - M S It alerted the Ex - Im Com panys, the in sp ect ion organs and the other related dep artm ents The iden tification m ethod is smi ple and speedy It can sp eed the cu stom s inspection and contribute to cross- stra it trades K ey W ords: T errap in Eggs; M ass Sp ectrogram of MALD I- TOF - M S; Pathogen ic Bacteria , , ,