Klebsiella Pneumoniae ECU-15生物纳米钯催化剂的制备、表征及其合成机理初探.pdfVIP

NO.4 Vo1.29 高校化学工程学报 第29 卷第4 期 2015 年 8 月 Journal of Chemica! Engineering of Chinese Universities Aug. 2015 文章编号: 1003-9015(2015)04-0873-08 Klebsiella Pneumoniae ECU-15 生物纳米钮催化剂的制备、 表征及其合成机理初探 雷彬，张旭，谭文松，朱明龙 (华东理工大学生物反应器国家重点实验室，上海 200237) 摘要2 利用自行筛选的克雷伯氏杆菌 ECU-15(Klebsiellapneumoniae ECU-15)还原钮离子制备了生物纳米钮催化剂， 并通过 TEM、 XRD、 EDS 对纳米钮的结构进行表征。 EDS 与 TEM 结果显示:纳米钮颗粒呈球形负载于菌体表面，平 均粒径为 15 nmo 0.5 mg 5% PdlPd(O)对 Cr(VI)(384 !1II10!.L-1)还原为 Cr(III)的转化率在 50min 内达到 99.67%。在此基础 上，进一步利用 FTIR 与氨基聚苯乙烯微球模拟实验推测κ pneumoniae ECU-15 合成生物纳米钮的可能机理是:微生 物细胞表面氢酶在生物纳米铝合成过程中调控Pd(O)晶体的活性位点，而表面氨基基团可能仅作为提供成核位点。该研 究结果可以为制备高效稳定的生物纳米钮催化剂提供了初步的理论基础和实践指导价值。 2 关键词:纳米钮:生物催化剂; Pd +还原; Cr(VI)还原 中图分类号: X78 1.1 文献标识码:A DOI: 10.3969/j .issn.1oo3-9015.2015.04.015 Preparation , Characterization and Synthesis Mechanism of Biopalladium Nanoparticles Produced by Klebsiella Pneumoniae ECU-15 LEI Bin, ZHANG Xu , TAN Wen-song , ZHU Ming-long (S饱te Key Laboratory ofBioreactor Engineering , East China University ofScience and Technology, Shanghai 200237 , China) Abstract: Palladium(Pd(O)) nanoparticles were prepared by Klebsiella pneumonia ECU-15 姐d th出 structural characteristics were analyzed with transmíssion electron microscopy(TEM) , X-ray difl企action(XRD) and energy dispersive spectrometer(EDS). TEM and EDS results indicate that the sphere-nano-palladium particles formed have an average size of 15 nm , which are mainly attached on cell surface. More than 99.67% of