雪松内生真菌的分离及抑菌活性研究.PDFVIP

安徽农业大学学报, 2012, 39(4): 619-623 Journal of Anhui Agricultural University 网络出版时间：2012-7-4 17:26:39 [URL] /kcms/detail/34.1162.S1726.012.html 雪松内生真菌的分离及抑菌活性研究 * 吴小文，郭正彦，柏 钰，花日茂 ，徐庆庆，吴祥为，李学德，唐 俊 (安徽农业大学资源与环境学院，安徽省农产品质量安全省级实验室，合肥 230036) 摘 要：采用组织分离法，从健康的雪松松针中分离纯化得到 11 株内生真菌。通过平板对峙和抑菌圈法将 11 株内生真菌分别对 10 株供试病原菌进行抑菌活性试验，结果显示内生真菌至少对 1 株供试病原菌存在抑菌活性。 内生真菌发酵液抑菌试验结果显示N03 菌株对苹果腐烂菌(Cytospora mandshurica)抑制率达到 80.56%，N06 菌株对 辣椒疫霉菌(Phytophthora capsici)抑制率达到 80.74%。N03 菌株不同天数的发酵液抑菌活性研究表明，发酵液的抑 菌活性与内生真菌的生物量相关。 关键词：雪松；内生真菌；抑菌活性 中图分类号：S476.19 文献标识码：A 文章编号：1672−352X (2012)04−0619−05 Endophytic fungi isolation from Cedrus deodara and their antifungal activities WU Xiao-wen, GUO Zheng-yan, BAI Yu, HUA Ri-mao*, XU Qing-qing,WU Xiang-wei, LI Xue-de, TANG Jun (Anhui Provincial Key Laboratory of Agro-Food Safety, School of Resources Environment, Anhui Agricultural University, Hefei 230036) Abstract: Eleven endophyte isolates were obtained from healthy needles of Cedrus deodara. The inhibitory activities of fermentation broth from 11 endophytes isolates against 10 pathogenic fungi showed that bioactivity of each endophyte against pathogenic fungus were different by a dual cultural method and inhibition zone method. The inhibitory activities of fermentation broth producing from strain N03 against Cytospora mandshurica reached 80.56%, and N06 strain against Phytophthora capsici reached 80.74%. The antifungal activities of N03 strain fermented with different days suggested that its antifungal activity was related to the biomass in fermentation broth of N03 strain. Key words: Cedrus deodara G; endophyte fungi; antifungal activity 植物内生真菌能够产生结构独特的代谢物。研 对细菌的抑制率达到 70%[5-6] 。国外已经开始了雪松 究发现内生真菌广泛分布于植物体根、茎、叶