超高效液相色谱-质谱对泻肺平喘灵水提物的成分研究.PDF

·530 · 国际中医中药杂志2017年6月第39卷第6期 Int J Trad Chin Med, June 2017, Vol. 39, No.6 ·实验与方药 · 超高效液相色谱-质谱对泻肺平喘灵 水提物的成分研究 王金华 王跃佳 巢建国 刘菊燕 【摘要】 目的 建立泻肺平喘灵水提物的超高效液相色谱-质谱(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS)检测方法，分析水提物主要成分。方法 采用UPLC-MS 法，通过与对照品的保留时间和质谱数据比对，鉴定部分成分；通过质谱数据分析，推测部分化合物结构； 流动相为乙腈-0.2% 甲酸水溶液，梯度洗脱；柱温30 ℃；流速0.8 ml/min；检测波长254 nm 。结果 从泻 肺平喘灵水提物中共检出 18 种成分，鉴定出盐酸麻黄碱、盐酸伪麻黄碱、丹酚酸 B 、大黄酸、芦荟大黄素、 大黄酚 6 种成分。结论 所建立的色谱方法稳定性高、重现性好，适于泻肺平喘灵水提物的分析。 【关键词】 泻肺平喘灵；水提物；超高效液相色谱-质谱 Study on main components of water extract in Xiefei-Pingchuanling using UPLC-MS * * Wang Jinhua , Wang Yuejia, Chao Jianguo, Liu Juyan. Gaochun Nanjing Hospital of Traditional Chinese Medicine, Nanjing 210023, China Corresponding author: Chao Jianguo, Nanjing University of Chinese Medicine, Nanjing 210023, China; Email: jgchaol016 @163.com 【Abstract 】 Objective To establish a Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS) chromatographic method for Xiefei-Pingchuanling extract, and to analyze the main components of its water extract. Methods The UPLC-MS was adopted with acetonitrile -0.2% formic acid aqueous solution (gradient elution) as mobile phase at a flow rate of 0.8 ml/min, detection wave length at 254 nm, and column temperature was 30 ℃. Results Eighteen components were separated from Xiefei- Pingchuanling extract, and eight ingredients were identified, such as the ephedrine, pseudoephedrine hydrochloride, salvianolic acidB, rhein, aloeemodin, chrysophanol, physcionwere. Conclusions The UPLC-MS chromatographic method has the sp