慢性给予皮质酮对小鼠抑郁样行为及突触素的影响.docxVIP

慢性给予皮质酮对小鼠抑郁样行为和突触素的影响孙秀萍1，杨久山2，徐艳峰1，徐玉环1，宋铭晶1，秦川1*（1.北京协和医学院比较医学中心中国医学科学院北京协和医学院实验动物研究所，卫生部人类疾病比较医学重点实验室，国家中医药管理局人类动物模型三级实验室北京100021；2.山东中医药大学附属医院，济南 250014）【摘要】目的通过饮入方式，慢性给予皮质酮，观察皮质酮对动物抑郁样行为和海马突触素的影响。方法c57雄性小鼠随机分为正常组和皮质酮组，每组10只。皮质酮组饮用含皮质酮的0.45%羟丙基-β-环糊精溶液，5 mg/kg/d。正常组动物给予0.45%羟丙基-β-环糊精溶液。造模35 d后，进行行为学实验。行为学实验结束后，动物灌注固定，应用免疫组化方法，检测突触素-1的表达。结果糖水偏爱测试结果表明，正常组和皮质酮组糖水偏爱指数无显著性差异（P0.05）。强迫游泳实验结果表明，皮质酮组强迫游泳不动时间显著延长（P0.05）。空场实验结果显示，正常组和皮质酮组总路程和速度无显著性差异。皮质酮组中央区停留时间、路程及进入中央区频次减少，边缘区停留时间增加（P0.05，P0.01，P0.01，P0.01）。新奇抑制摄食实验结果表明，皮质酮组摄食潜伏期显著延长（P0.05），5 min内摄食量无变化。免疫组化结果显示，皮质酮组突触素-1表达显著下降（P0.05）。结论通过饮入方式，慢性给予皮质酮，动物出现抑郁样表现，可能与海马突触素-1表达减少，神经突触传递效能降低有关。【关键词】皮质酮；抑郁；突触素；小鼠The effect of chronic corticosterone administration on depressive-like behaviour and synapsin-1 expression in miceSUN Xiu-ping1, YANG Jiu-shan2,XU Yang-feng1,XU Yu-huan,,SONG Ming-jing1,QIN Chuan1*(1.Comparative Medicine Center，Peking Union Medical College(PUMC)，and Institute of LaboratoryAnimal Science，Chinese Academy of Medical Sciences(CAMS)；Key Laboratory of Human DiseaseComparative Medicine，Ministry of Health；Key Laboratory of Human Disease Animal Models, State Administration of Traditional Chinese Medicine，Beijing 100021，China；2.Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Jinan，250014)【Abstract】Objective To investigate the effect of chronic corticosterone administration on depressive-like behaviour and synapsin-1 expression in mice. Method C57BL/6Jmice were randomly separated into 2 groups (n = 10 per group): control and Corticosterone( CORT).CORT was dissolved in vehicle(0.45%hydroxypropy-β-cyclodextrin,β-CD).Corticosterone(5mg/kg/day)wasdeliveredfor 35 daysindrinkingwaterandcontinuedwhenthebehavioraltestswereperformed. Controlanimalsreceivedvehicle(β-CD) indrinkingwater duringtheentireexperiment. The behavior tests were applied to evaluate depressive-like behavior of the model. ResultsThe sucrose preference test showed no significant difference in sucrose intake between control