MALDI-TOF质谱技术分析与鉴定病原细菌研究.PDF

微生物学通报 MAR 20, 2009, 36(3): 416~426 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究快报 MALDI-TOF 质谱技术分析与鉴定病原细菌研究 1,2 2* 2 1 2 2 其布勒哈斯 田世民 邹明强 贺银凤 陈 艳 赵贵明 (1. 010018) (2. 100123) 摘 要: 本文通过基于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术分析病原细菌 的方法进行研究, 阐明影响分析结果的重要因素, 并建立了 MALDI-TOF-MS 分析病原细菌的标 准方法。对不同属、种和亚种的 12 株植物病原细菌进行全细胞分析结果表明：MALDI-TOF-MS 能快速而准确的区分和鉴定病原细菌, 分析过程简单、灵敏度高。此法在细菌属、种、亚种和菌 株水平上, 可快速、准确地区分和鉴定。 关键词: MALDI-TOF MS, 细菌质量指纹图谱, 鉴定, 病原细菌 Analysis and Identification of Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Qibulehasi1,2 TIAN Shi-Min2* ZOU Ming-Qiang2 HE Yin-Feng1 CHEN Yan2 ZHAO Gui-Ming2 (1. Inner Mongolian Agricultural University , Hohhot, Inner Mongolia 010018, China) (2. Chinese Academy of Inspection and Quarantine, Beijing 100123, China) Abstract: Based on Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI- TOF-MS) method for discrimination of pathogenic bacteria was developed. Established the standardization method for MALDI-TOF-MS analysis of bacteria and affecting elements of the analyzing result. The results obtained from twelve plants pathogenic bacteria of different genus, species and subspecies were analyzed by using MALDI-TOF showed that, MALDI-TOF-MS could be rapid and reliable identification of bacteria at the level of genus, species, and strains. Analytical process is simple, high sensitivity are advantages of this strategy. Keywords: MALDI-TOF-MS, Bacteria mass finger-printing, Id