调节性T细胞治疗实验性小鼠多发性肌炎和机制研究.PDFVIP

·602 · doi 10.3969/j.issn. 1673-4254.2015.04.29 J South Med Univ, 2015, 35(4): 602-605 基础研究 调节性调节性TT 细细胞治疗实验性小鼠多发性肌炎及机制研究胞治疗实验性小鼠多发性肌炎及机制研究 1 1 2 2 石 强 ，田成林 ，刘洁晓 ，蒲传强 1 2 中国人民解放军总医院海南分院神经内科，海南 三亚 572000 ；中国人民解放军总医院神经内科，北京 100853 摘要：目的探讨CD4+ CD25+ Foxp3+ Treg 细胞对于实验性自身免疫性肌炎（EAM）小鼠的治疗价值及机制。方法 免疫磁珠分 选技术从BALC/c 小鼠脾脏中分选出足量CD4+ CD25+ Foxp3+ Treg 细胞以备回输，观察干预组及未干预组EAM 小鼠肌肉组织 病理学变化，流式细胞仪检测两组小鼠脾脏CD4+ CD25+ Foxp3+ Treg 细胞表面PD- 1及CTLA-4 的表达变化，双抗体夹心ELISA 法检测外周血IL- 10、TGF-β的变化。结果 干预组小鼠较未干预组肌肉病理炎性细胞浸润明显减轻，其外周血IL- 10、TGF-β含 量较未干预组明显升高（P0.05），脾脏CD4+ CD25+ Foxp3+ Treg 细胞表面PD- 1及CTLA-4 表达明显升高（P0.05）。结论CD4+ CD25+ Foxp3+ Treg 细胞回输对于EAM 小鼠的治疗效果是通过增加外周血IL- 10及TGF-β水平和增高脾脏CD4+ CD25+ Foxp3+ Treg 细胞表面PD- 1及CTLA-4 的表达发挥作用。 关键词：自身免疫性肌炎；CD4+ CD25+ Foxp3+ Treg 细胞；流式细胞仪；IL- 10；TGF-β；PD- 1；CTLA-4 Regulatory T cells in the treatment of autoimmune myositis in mice: efficacy and mechanism 1 1 2 2 SHI Qiang , TIAN Chenglin , LIU Jiexiao , PU Chuanqiang 1Department of Neurology, Hainan Branch of General Hospital of PLA, Sanya 572000, China; 2Department of Neurology, General Hospital of PLA, Beijing 100853, China Abstract: Objective To investigate effect of CD4+ CD25+ Foxp3+ Tregs in the treatment of autoimmune myositis (EAM) in mice and explore the possible mechanisms. Methods Mouse models of EAM were divided randomly into model group and treatment group, and the latter received infusion of CD4 + CD25 + Foxp3 + Tregs separated from normal mouse spleen by magnetic activated cell sorting. The changes of muscle pathology was observed, and the expression of PD-1 and CTLA-4 in spleen CD4 + CD25 + Foxp3 + Tr