朱红梅assembly0422.pdfVIP

Genome Assembly Part I: Why is it difficult and what are the general strategies Reads from sequencing platform 1. Reads start at random positions on the genome 2. Coverage = (overall reads length) / (genome length) Sequence overlaps are the main foundation for assembly 1. “Real” overlap 2. “False” overlap overlap by coincidence ATTGATC ATTGATC ATTGA ATTGA Assembly difficulty from repeats 1. Repeats in genome might cause mis