源自合肥地区表观健康鸡的沙门氏菌耐药性和血清型、基因型.PDF

安徽农业大学学报, 2013, 40(4): 540-546 Journal of Anhui Agricultural University 网络出版时间：2013-7-8 18:08:35 [URL] /kcms/detail/34.1162.S1808.007.html 源自合肥地区表观健康鸡的沙门氏菌耐药性与血清型、 基因型相关性分析 王元兰，刘军军，邵 颖，方艳红，魏建忠，王桂军，孙 裴，李 郁* (安徽农业大学动物科技学院，合肥 230036) 摘 要：为了解源自合肥地区表观健康鸡的沙门氏菌耐药性及其与血清型、基因型的相关性，在前期已鉴定血 清型和ERIC 基因型的基础上，采用K-B 纸片法对21 株沙门氏菌分离株进行21 种抗生素药物敏感试验，利用PCR 技术对耐药菌株进行32 种耐药基因检测。结果显示，总耐药率为100%（21/21 ），耐2 种以上抗生素的菌株占76.19% （16/21 ），多重耐药谱为青霉素-氨苄西林-阿莫西林-链霉素-复方新诺明，其中耐受β- 内酰胺类抗生素的类别最多， 耐药率最高达100% （21/21 ）。21 株沙门氏菌均检出耐药基因，涉及blaPSE 、sul1 、strA 、blaTEM 、sul2 、tetA 、tetB 、 aph3- Ⅱa 和cat Ⅰ 9 种，其中blaPSE 基因的检出率最高，为85.7% （8/21 ）。可见源自合肥地区表观健康鸡的沙门氏 菌对β- 内酰胺类抗生素耐药性最强，与其携带blaPSE 基因有关；耐药表型测定结果与耐药基因检测结果基本一致； 耐药性与具有相同来源的同一血清型、基因型相关。 关键词：表观健康鸡；沙门氏菌；耐药性；血清型；ERIC 基因型 中图分类号：S858.31 文献标识码：A 文章编号：1672−352X (2013)04−0540−07 Analysis of antimicrobial resistance on Salmonella isolated from nonsymptomatic chickens in clinic in Hefei and its serotype and genotype WANG Yuan-lan, LIU Jun-jun, SHAO Ying, FANG Yan-hong, WEI Jian-zhong, WANG Gui-jun, SUN Pei, LI Yu (School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036) Abstract: In order to investigate the relationship between antimicrobial resistance and serotypes, genotypes of Salmonella isolates from apparently healthy chickens in Hefei, the Kirby-Bauer method was applied to deter- mine the sensitivity of 21 strains susceptibility to 21 antibiotics, and PCR technique also was used to detect 32 kinds of drug-resistance genes, based on identified serotypes and ERIC-PCR gene-type. It was found that 100%(21/21)of the Salmonella isolates were resistant to all antibiotics by Drug Susceptibility Test; 76.19% (16/21) were resistant to at least two antimicrobials