海岛棉GbPR10-1基因的克隆和功能分析.pdfVIP

硕士论文 ABSTRACT CLONING AND CHARACTERIZATION OF GbPR10-1 GENE OF SEA-ISLAND COTTON (GOSSYPIUM BARBADENSE) ABSTRACT The great loss of cotton is majorly due to the infection of verticillium wilt. When infected with verticillium pathogens, the cotton usually produces a series of resistance signals, among which, pathogenesis-related protein family 10 is closely related to resistance response. One full-length cDNA of PR10 gene, named GbPR10-1 （GenBank Accession AY241395 ）, was isolated from the established cDNA library of Gossypium barbadense root infected with Verticillium dahliae. GbPR10-1 was 745 bp with a 91 bp 5’ UTR and a 177 bp 3’ UTR. The GbPR10-1 had an open reading frame of 477 bp, encoding a predicated polypeptide of 159 amino acids with a calculated molecular weight of 17.9 kDa and pI of 4.95. The deduced GbPR10-1 protein contained a conserved domain K-A-X-E-X-Y-L in the C-terminal helix, but the typical glycine-rich P-Loop structute, G-X-G-G-X-G, was variable in GbPR10-1. Genomic Southern blot analysis indicated that the GbPR10-1 belonged to a low copy gene family in G. bardadense. The expression profile using RT-PCR analysis, demonstrated that the transcript level of GbPR10-1 was elevated when the plant was stimulated by heat and salt, but not by ABA. The bacterially expressed GbPR10-1 protein showed the low ribonuclease activity in vitro and inhibited the growth of Verticillium iv 硕士论文 ABSTRACT dahliae hyphae. In the