牙鲆变态过程中三种碘化甲状腺氨酸脱碘酶基因的时空表达.pdfVIP

  检测到了 Dio1 和 Dio3 的信号，并且在Dio1 基因在 G 期几乎整条侧线均有信号， Dio3 基因在 F 期信号几乎遍布整条侧线。在Dio3 的变态期的结果中发现，在肠 道，心脏，肌肉，眼睛等组织的外表皮有实心点状信号，这种信号在 Dio1 和 Dio2 的检测结果中均没有发现。 总之，本论文的研究结果：Dio1 ，Dio2 和 Dio3 在牙鲆变态过程中的时空分 布既有相同点又有不同之处，这三种脱碘酶 RNA 整体原位杂交图式的给出，为 后面研究这三种基因在牙鲆变态过程中作用提供了基础。 关键词：牙鲆；变态；1 型甲状腺氨酸脱碘酶；2 型甲状腺氨酸脱碘酶；3 型甲 状腺氨酸脱碘酶；RNA 整体原位杂交；眼睛移动 II      The Spatio-temporal Expression Pattern of Three Iodothyronine Deiodinases during Metamorphosis of Paralichthys olivaceus ABSTRACT Flatfish （Pleuronetiform ）metamorphosis is the most evident considering its transition from symmetrical pelagic larvae to unsymmetrical benthic juvenile, with one eye migration from one side to another, pigment occurence on the ocular side, the change of digestive tract, bone remodeling, and the nsynchronous development of paired fins. Thyroid hormone is necessary for most vertebrates, and the metamorphosis of flatfish is also regulated by thyroid hormone. T4 is the main product of thyroid with little biological activity. In order to produce T3, which has considerable biological activity, T4 molecule deiodination should happen at the outer ring, which was catalyzed by type1 iodothyronine deiodinase(Dio1) and type 2 iodothyronine deiodinase(Dio2).If the deiodination happens at the inner ring of T4, the rT3 will be produced, which has no biological activity. The inner ring deiodination of T4 was catalyzed by type 3 iodothyronine deiodinase(Dio3), which can also deiodinate T3 and thereby terminate thyroid hormone action. Three deiodinases play roles in maintaining thyroid hormone homeostasis, at the same time, they also participate in local thyroid hormone controlling. Although it is almost clear tha