特应性皮炎患者皮损致病真菌定植分析.pdfVIP

特应性皮炎患者皮损致病真菌定植分析 硕士研究生：张秀钦 导 师：程波 教授 中 文 摘 要 目的: 观察50 例AD 患者念珠菌、红酵母、青霉、曲霉这4 种常见致敏真菌在 AD 患者皮肤表面定植情况，分析致敏真菌在AD 发病中的作用。 方法：研究对象为 2012 年6 月至 2012 年12 月间在福建医科大学附属第一医 院皮肤科就诊的特应性皮炎患者共50 例，中度与重度患者各选25 例，20 例健康 对照者，所有入选患者两周内均未使用抗真菌药、糖皮质激素或免疫抑制剂系统及 外用治疗，分别对其皮损部位及非皮损部位采用常规真菌培养方法，将标本接种于 沙保罗培养基，置25℃恒温箱内培养；发现真菌及酵母样可疑菌落，转种沙保菌 斜面，获得纯培养后鉴定， 根据菌落形态特征及颜色、菌落生长的快慢、镜下孢 子及菌丝的特征鉴定。采用 SPSS 13.0 统计软件，用χ2 检验分析阳性率差异的显 著性，P＜0.05 为差异有统计学意义。 结果：AD 患者皮肤表面念珠菌、红酵母检出率显著高于正常对照组，且重度 患者检出率高于中度患者；青霉、曲霉检出率与正常对照组相比无统计学意义。 结论：AD 患者皮肤表面念珠菌、红酵母定植明显高于正常对照组，并且与AD 患者症状的严重程度相关。 关键词：特应性皮炎，念珠菌，红酵母，真菌培养 5 Analysis of pathogenic fungal colonization on skin lesion of atopic dermatitis patients Postgraduate, Zhang Xiuqin Tutor, Prof . Cheng Bo Abstract Objective : To investigate the colonization of four common sensitizing fungi Candida, Rhodotorula, Penicillium, Aspergillus on the skin surface of AD patients, and to analyze sensitization fungi in the role of the AD. Methods : The study enrolled a total of 50 patients with atopic dermatitis (moderate: n=25, severe : n=25) from June 2012 to December 2012 at Dermatology Department of the First Affiliated Hospital of Fujian Medical University, 20 healthy subjects served as controls. All enrolled patients did not use antifungal agents, glucocorticoids or immunosuppressants and topical treatment within two weeks before enrollment. Conventional fungal culture of lesional and non-lesional skin specimens were inoculated in the Sabourands Medium, and cultured at a constant temperature of 25 ºC. If suspicious fungi and yeast-like colonies were discovered, the colonies were subcultivated for pure culture identification, according to morphologic characteristics and color of colony, colony growth speed and