空心菜青枯病病原菌的鉴定.pdfVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 38(2): 120幡125(2ω8) 空心菜青枯病病原菌的鉴定 何自福1 ，余小没1 气虞蜡1 ，罗方芳1 ，李华平2 e广东省农业科学院植物保护研究所，广州 51师40; 悻南农业大学资源环境学院，广州 5H刷2) 摘要:空心臻青柑病最近年来在广东空心菜产阪新发生的病害，病株率一般为 5% -20% ，严重达501岛以上。症状初始表 现为樵株上部1-2 片叶赘萧、下嚣，病叶失去光泽黛灰绿色;随着病情的发展，病叶不断增多，最终接株萎麓，甚至倒伏。 经细菌学接定、室内致病性测定、16S rDNA 序列比较及系统进化分析，结果表明引起该病害的病原是茄科雷尔氏商 (Ralsωnia solatUlCearum) ; 致病性和碳水化合物利用试验结果表明，该病原菌属茄科雷尔氏菌 l~亲生理小种，生化变种40 关键词:空心菜青柑病;茄科雷尔氏酶;病原鉴定 Pathogen identification of bacterial wilt of Ipomoea aquatica HE Zi也 SHE Xiao~ 2 man.2 , YU Hao , LUO Fang.腼 fang ; LI Hua帽ping ( 1Institute of Plant Protection , Guangdong Academy of A回cultural Sciences , Guangzhou 51阳40 ， China; 2College of Nature Resources and Environment , South China Agricultural University , Guangzhou 51侃42 ， China) Abstract: 机le bacterial wi1 t of 非omoea aquatica is a new disease ín Guangdong province in recent years. 叽le incidenc咽 of diseased plants were 5% - 20% in the fields , even rnore than 50%. Initially , one or two upper leaves of the díseased plants wilted and became gray~green. Along with the disease developing , the di~ 盹ased leaves of the plants increased. And finally. the whole plants completely wilt时， even col1apωd. By using bacteriological identification , pathogenicity tests , 16S rDNA sequence comparison and phylogene伽rela­ tionships analysis , the bacterial wi1t of 1. aquαtica was caused by Ralstonia solanacearum. The results of pa由， ogenicity and carbohydrate uti1ized test demonstrated that the tested strains of the pathogen belonged ωrace 1 and biovar 4 of R. so切nacearum. Keγwords: bacterial wilt of Ipomoea αquatica; Ralstonia solanacearum; pathogen identifying 中回分提号:制36.32 文章编号: 0412-0914(2佣8)02-û120船 文献栋i只码:A 空心菜(Ipomoeα 叫ωtica Forsl屿，又称署在荣、 害发