防风镇痛成分--CPB--2001.pdfVIP

154 Chem. Pharm. Bull. 49(2) 154—160 (2001) Vol. 49, No. 2 Analgesic Components of Saposhnikovia Root (Saposhnikovia divaricata) Emi OKUYAMA,* Tetsuya HASEGAWA, Takamitsu MATSUSHITA, Haruhiro FUJIMOTO, Masami ISHIBASHI, and Mikio Y AMAZAKI Faculty of Pharmaceutical Sciences, Chiba University, 1–33 Yayoicho, Inage-ku, Chiba 263–8522, Japan. Received August 18, 2000; accepted November 7, 2000 By activity-oriented separation using the writhing method in mice, the analgesic components of Saposh- nikovia root (Saposhnikovia divaricata Schischkin; Umbelliferae) were identified to be chromones, coumarins, polyacetylenes and 1-acylglycerols. Two new components, divaricatol and (3S)-hydroxydeltoin, were also isolated. The most potent analgesia was observed in chromones such as divaricatol, ledebouriellol and hamaudol, which inhibited writhing inhibition at an oral dose of 1 mg/kg in mice. Acylglycerols also showed inhibition significantly at a dose of 5 mg/kg. In some pharmacological tests using sec-O-glucosylhamaudol, the compound showed analgesia by the tail pressure and the Randall Selitto methods, and its writhing inhibition was not reversed by naloxone. Key words Saposhnikovia divaricata; analgesia; divaricatol; (3S)-hydroxydeltoin; chromone; 1-acylglycerol In view of their scientific understanding and potential dose of 3 g/kg. The separation of the extract was mainly car- usage, we have continuously investigated traditional medi- ried out by following the writhing inhibition in mice with cines to clarify the active components and their pharmaco- oral administration of samples as an