鹅生长激素基因的克隆和组织表达.pdfVIP

业生物技术学报 Journal of Agricultural Biotechnology 2004,12 (5): 552~555 ·研究论文· 鹅生长激素基因的克隆和组织表达* 敖金霞 李 辉** 王启贵 王宇祥 唐志权 (东北农业大学动物科学技术学院，哈尔滨150030) 摘要：根据鸡的生长激素基因( )序列设计引物对鹅总RNA 进行RT-PCR 反应，将扩增片段进行克隆和测序。结果获得 了包括从ATG 开头到TGA 结尾的651 bp 的鹅 基因编码区序列。鹅与鸡的 基因的编码区高度保守，同源性达92% ，演 译成氨基酸后同源性达96% ；与人和鼠 基因编码区序列同源性分别为63.81%和74.3 1% ，演绎成氨基酸后同源性分别为 52.51%和72.81%。同时用RT-PCR 和Northern-blot 对鹅 12 组织表达差异分析表明鹅 基因只在垂体和下丘脑中表达，在 心脏、肺、肝脏、小肠、胃、腿肌、脾、肾脏、脂肪和睾丸中都不表达。 ： ；克隆；组织表达 关键词 鹅；生长激素基因 Cloning and Tissue Expressions of Growth Hormone Gene in Goose AO Jin-Xia LI Hui** WANG Qi-Gui WANG Yu-Xiang TANG Zhi-Quan (College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030 ，China) The primers were designed according to the sequence of chicken growth hormone ( ) gene and PT-PCR method was developed to amplify the goose RNA. Then PT-PCR product was cloned and sequence. The 65 1 bp coding region sequence from ATG to TGA of the goose gene was obtained. Homologous comparison showed that the identity of of gene was 92% between goose and chicken, and that was 96% in amino acid sequences. Further analysis showed that the identities of of goose GH gene with human and mouse were 63.81% and 74.31% and that were 52.51% and 72.81% in amino acid level, respectively. RT-PCR and Northern-blotting analysis in 12 kinds of tissues indicated that the goose gene expressed in pituitary and thalamus and did not express in the heart, lung, liver, intesti