院感常见菌监测及阴沟肠杆菌耐药基因分型.pdfVIP

Abstract ABSTRACT Objective Antibiotic resistance has been a puzzled global problem in the treatme nt of clinical infection for long time. The species, distribution and drug resistance of clinical common bacteria varied in different hospital of the different region, which result from different routine usage of antibiotics and their frequencies. Therefor e, long-term monitoring the resistance of clinical common bacteria have practical significance in guiding and adjusting the usage of antibiotics in different hospitals. This subject aims at: Monitoring the species, distribution and drug resistance of clinic common bacteria in one of the hospital in Ganzhou city, exploring the related drug resistant genes in Enterobacter cloacae. These study results will be beneficial to guiding the clinical antibiotic choice in the hospital in Ganzhou city, and will provide theoretical foundation for monitoring the new mutant Enterobacter cloacae strains. Materials and methods Isolated clinical bacteria from one of the hospital in Ganzhou city druing Jan. 2013 to Apr. 2014, and identified all the strains by Vitek2 compact automatic microbe analytic instrument. 40 Enterobacter cloacae strains were collected and their minimal inhibitory concentrations(MICs) to common antibacterial agents were detected and then analysed according to CLSIM100-S24 (2014). Plasmid DNA of Enterobacter cloacae strains were extracted with alkaline lysis method. 12 kinds of genotype were detected by polymerase chain reaction （PCR ）technic. Results 1. Clinic strains were isolated most frequently from sputum (41.56%), urinary surgery （30.14% ） and respiration medicine （22.18% ）are the main sources.