大孔树脂法分离二乔木兰花红色素-食品科学.PDF

※工艺技术 食品科学 2007, Vol. 28, No. 09 235 1 1 1 2 唐克华 ，戴兰芳 ，唐万勇 ，于华忠 (1.吉首大学城乡资源与规划学院，湖南 张家界 427000； 2.吉首大学 林产化工工程湖南省重点实验室，湖南 张家界 427000) ：探讨了八种大孔树脂对二乔木兰花红色素组分的分离性能，并进行了色素成分的高效液相色谱(HPLC) 法分离。研究表明：LD-607 树脂对二乔木兰花红色素的纯化较为适宜，其对该花色素制备样液的吸附容量达 23.8ml/g 干树脂，并可使样液中色素浓度提高为原来的8倍左右。HPLC法分离结果表明，在二乔木兰花红色素的 218、329nm 两组分中，每种组分均至少含两种色素成分。 ：二乔木兰；花；色素；树脂；分离 Separation of Red Pigments from Magnoliaceae ×soulangeana Flower by Macroporus Resin Column Chromatography 1 1 1 2 TANG Ke-hua ，DAI Lan-fang ，TANG Wan-yong ，YU Hua-zhong (1.College of Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China； 2.Key Laboratory of Forest Product and Chemical Engineering of Hunan Province, Jishou University, Zhangjiajie 427000, China) Abstract ：The adsorption and desorption performance of eight macroporus adsorbent resin for separation red pigments from Magnoliaceae ×soulangeana flower, and determination of pigments by high pressure liquid chromatography(HPLC) were studied. The results showed that the LD-607 is optimum for absorbing pigments among the eight resins, and its adsorption capacity for the liquid pigments sample is 23.8 ml/g dry resin. The concentration of pigments in the liquid sample is eight times of the first concentration after the pigments separated from the sample. In pigments of 218 nm or 329 nm, more than two kinds of constituents are discovered by HPLC analysis. Magnoliaceae ×soulangeana；flower；pigments；resin；separation Key words： 中图分类号：O652.63；R284.2