萝卜及其芽苗中异硫氰酸酯分布探究.docVIP

萝卜及其芽苗中异硫氰酸酯分布探究 　　摘要：利用二乙胺滴定法和硫脲分光光度法测定并比较了萝卜及其芽苗各部位中异硫氰酸酯的含量。结果表明，萝卜及其芽苗不同部位中异硫氰酸酯的含量明显不同。萝卜从头到中到尾3个部位异硫氰酸酯的含量依次增大；萝卜尾部皮部中异硫氰酸酯含量要高于肉质部；萝卜芽苗叶中异硫氰酸酯的含量高于茎；二乙胺滴定法测定结果明显优于硫脲分光光度法。二乙胺滴定法和硫脲分光光度法测定萝卜及其芽苗中的异硫氰酸酯操作简单、成本低廉，结果理想 关键词：异硫氰酸酯（ITCs）；二乙胺滴定法；硫脲分光光度法；萝卜；萝卜苗 中图分类号：S631.2；Q946.4 文献标识码：A 文章编号：0439-8114（2016）21-5631-03 DOI：10.14088/j.cnki.issn0439-8114.2016.21.050 Research on the Distribution of Isothiocyanates in Radish and Sprout ZHAI Shu-hong1，LIU Qing-pei2，LI Jie2 （1.Wuhan Institute of Design and Sciences，Wuhan 430205，China； 2.College of Food Science and Technology，Huazhong Agricultural University，Wuhan 430070，China） Abstract：The isothiocyanates contents in the different parts of radish and its sprout were determined and compared by diethylamine titration method and thiourea spectrophotometry. The results showed that there were obviously different isothiocyanates content in the different parts of radish and its sprou. The content of isothiocyanates in the three parts of radish were successively increased from head， middle to tail of radish. The content of isothiocyanates in the tail and skin were higher than that in the flesh part of radish. The content of isothiocyanate in sprout was higher than that in stem. The conclusion showed the diethylamine titration method was obviously superior to the thiourea spectrophotometry. The diethylamine titration method - thiourea spectrophotometry was simple， inexpensive and effective. Key words： isothiocyanates； diethylamine titration method； thiourea spectrophotometry； radish； radish sprouts ?}卜为十字花科萝卜属根菜类蔬菜，具较很高的食用和保健价值。异硫氰酸酯（Isothiocyanates，ITCs）是一类易挥发、具催泪性、有刺激辣味的物质，是十字花科蔬菜中大量存在的硫苷酶解产物，也是萝卜呈辣的主要组分，具有高度的生物学活性，有杀菌、解毒、抑制血小板聚集、抗癌症等作用[1-3]。异硫氰酸酯类化合物也具有一定的毒副作用，对人的嗅觉、味觉以及甲状腺等有刺激，过多地食用含此类物质的食品会给人的身体健康带来一定的危害。因此，准确地测定萝卜中异硫氰酸酯的含量是十分必要的 本研究采用二乙胺滴定法和硫脲分光光度法测定萝卜及其芽苗中的ITCs含量，比较萝卜及其芽苗不同部位ITCs差异，为萝卜中ITCs的研究和开发利用提供参考 1 材料与方法 1.1 植物与试剂 萝卜，华中农业大学菜市场售；萝卜芽苗，实验室自制。盐酸、无水磷酸氢二钠、十二水合磷酸氢二钠、柠檬酸、抗坏血酸、无水乙醚、无水乙醇、无水硫