中药调整性早熟儿童青春发育进程的机制研究论文.docVIP

　　中药调整性早熟儿童青春发育进程的机制研究论文 蔡德培，陈伯英，张炜，李嫔 【关键词】 ,中药 ［摘要］ 目的：从神经内分泌调节及基因表达的角度，探讨调整性早熟儿童青春发育进程的滋阴泻火中药和益肾填精中药有效调节下丘脑垂体促性腺机能、促生长机能的作用机制。方法：青春期大鼠分别予以喂饲滋阴泻火中药或益肾填精中药。采用神经生物学实验方法（下丘脑推挽灌流、组织匀浆、脑片孵育及免疫组化法）检测下丘脑促性腺区促性腺激素释放激素（gonadotropinreleasing hormone, GnRH）的含量、脉冲释放频率与幅度，以及中枢氨基酸递质、神经肽Y及β内啡肽释放的变化；采用定量逆转录聚合酶链反应检测下丘脑GnRH、生长激素释放激素（groone releasing hormone, GHRH）、生长抑素（somatostatin, SS）、腺垂体卵泡刺激素（follicular stimulating hormone, FSH）、黄体生成素（luteinizing hormone, LH）、生长激素（groone, GH）、长骨干骺端胰岛素样生长因子Ⅰ（insulinlike groedicine on modulating the course of puberty development in children atotrophic functions of hypothalamicpituitary axis, and to reveal the mechanisms of ZYXHR and YSTJR in modulating the course of pubertal development of children eters of rats onitored as the folloone（GnRH）, the frequency and amplitude of GnRH impulse releasing, the releasing amounts of aminoacid neurotransmitters,.freelus ethods (pushpull perfusion, homogenate, incubation of brain slices, and immunohistochemical staining). The levels of gene and protein expressions of GnRH, groone releasing hormone (GHRH) and somatostatin (SS) in hypothalamus, and follicular stimulating hormone (FSH), luteinizing hormone (LH) and groone (GH) in adenohypophysis as etaphysis ined e reverse transcriptionpolymerase chain reaction (RTPCR). Results: The ZYXHR could reduce the activity of GnRH neurons in hypothalamus through inhibiting the release of central exciting aminoacid neurotransmitters, oting the release of central inhibiting aminoacid neurotransmitters, NPY and βEND in gonadotrophic area of hypothalamus. The expression levels of GnRH, FSH and LH mRNAs RNA in hypothalamus RNA in hypophysis and the IGFⅠ mRNA in metaphysis ote the activity of GnRH neurons in hypothalamus through inhibiting the release of NPY in gonadotrophic area of hypothalamus, upregulating the expression levels of GnRH, FSH, LH and GH mRNAs in hypophysis, and IGFⅠ mRNA in metaphysis, RNA in hypothalamus. Conclusion: The ZYXHR and YSTJR could both regulate