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三倍体欧洲山杨快速繁殖体系的建立论文
Abstract
an
isoneof industrialtree,Thereare researchestissue
Populus important many about culture
and of onnative stemsections
rapid Populus,mostly of
propagation focusing variety,Using
as material.Weonthe
European
triploid aspentesting study influencingfactors,samplingtime,
and included.A
sterilization,rapidpropagation,rootingtransplant preliminaryrapid
of is itisof forthe
systemtriploidEuropean established,and
aspen greatsignificancerapid
andextensionoffinetree.
propagation
Inmid weusebiennialstemsectionsas culturetheminwetsand.When
April material,and
buds cut andlaminaeas
growbigenough,we
offgrowingpoints、petioles explants.In
is in waterfor10 scoursedthemfor3hoursin
processstep,explantput soapy minutes,next
themin75%alcoholand0.1%mercuric for
water,then chloride 30seconds
running dip separately
and6 lastusesterilizationwater to
minutes,at them3 5times.Thesteriliztoneffecfsis
washing
onthe of mediumandthekindsandconcentrationof
900d.Westudy typeexplants、basic plant
resultsshowthatthe rate in
growthregulator,the is cultivatedMS
highestindu
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