硕士论文英文摘要.docVIP

帮忙改改英文摘要吧摘?要目的及意义：大面积皮肤创伤需要通过皮肤或人工皮肤移植来实现创面修复。本研究旨在研发一种含有生物活性因子的可降解纳米纤维膜来有效修复创面，满足市场需求。研究内容和方法：1．?应用高压静电纺丝技术、静电电喷技术分别构建聚己内酯纤维膜、聚己内酯/明胶复合纤维膜、聚己内酯/明胶/生物活性因子纤维膜。通过称重法、扫描电镜、衰减全反射-傅里叶变换红外光谱、静态接触角、降解率测试等试验对三种纤维膜表征进行检测。2．?通过细胞学实验、动物实验检测三种纤维膜细胞相容性及其创面修复效果。结果：1．?表征测试显示PCL、PCL/GE、PLC/GE/BF三种膜孔隙率由高到低依次为：PCL膜＞PLC/GE/BF＞PCL/GE膜，密度由高到低依次为：PCL/GE膜＞PLC/GE/BF＞PCL膜,?三组膜具有低密度和高孔隙率的特性，纤维表面光滑。2．?体外降解实验显示纤维中明胶可以增加PCL的亲水性，而且加速了纤维的降解过程。3.??细胞纤维膜共培养结果显示PCL/GE/BF、PCL/GE膜具良好的细胞黏附性及细胞相容性，PCL/GE/BF上细胞增殖最为明显，PCL膜上细胞增殖效果较前两者差。4.??新西兰兔创面修复实验及病理切片显示PCL/GE/BF有利于创面毛细血管早期形成，PCL/GE/BF明显的促进创面修复效果，创面有良好的保护作用PCL/GE膜对创面保护作用强于PCL膜。结论：PCL/GE/BF作为可降解支架具有良好的生物相容性，在促进创面修复方面具有巨大优势。关键词：明胶;?聚己内酯;?生物活性因子;?生物相容性;?组织工程 A?large?area?of skin?wounds?to achieve wound healing by the skin or artificial skin graft. The purpose of this study to develop a containing biological active factor biodegradable nanofibrous membrane which can be used to wound repair effectively and meet?the?market?demand.Contents?and?Methods1.?The?PCL?fiber?membrane,?PCL/GE?fiber?membrane?and?PCL/GE/BF?membrane?were?made?by?high voltage electrostatic spinning technology and electrostatic?spray?technology respectively. The three kinds of fiber membranes were characterized by?weight?measurement, SEM,ATR-FITR, Static?contact?Angle?(SCA),?degradation?test. The cell compatibility and wound healing effect of three kinds of fiber membrane were?studied?by cytological and animal experiments. Results ?Characterization?tests?revealed that?the?porosity?of?three?membranes?in order was:?PCL?film??PCL/GE/BF??PCL/GE?film,?and the order of the three membranes density was:?PCL/GE?film??PCL/GE/BF??PCL?film, Three groups of membrane with low?density?and?high?porosity characteristics, and?the?surface?of?fibers?were?smooth. The?degradation?experiment?in?vitro?showed?that?gelatin?was added into?fiber?can not?only?increase?the?PCL?hydrophilic, but?also?accelerate the rate of fiber?degradation. Cell?scaffold?co-culture?tests showed that PCL / GE /