常用生化检测项目分析方法举例及参数设置(国外英文资料).doc

常用生化检测项目分析方法举例及参数设置(国外英文资料) Examples and parameter setting of common biochemical analysis items Recommend a, commonly used biochemical testing project analysis methods for example 1. end point detection method commonly used with total bilirubin (oxidation or heavy nitrogen), total bilirubin (oxidation or diazotization method), serum total protein (biuret method), serum albumin (bromocresol chloride method), total bile acid (enzymatic), glucose (glucose oxidase, uric acid (UA) enzymatic), total cholesterol (cholesterol oxidase), triglycerides (glycerol phosphate oxidase method), high density lipoprotein cholesterol (direct method), calcium (Arsenazo III method), phosphorus (UV) and magnesium (two toluidine blue method). In the project, in addition to calcium, phosphorus and magnesium basically also use it with a single reagent of end point method, determination of other projects can use double reagent so it can choose two end point method, including total protein and albumin were measured for two reagents available. Uses this method to measure creatinine 2. fixed time method of picric acid method. General for determination of enzyme activity using continuous monitoring method 3. continuous monitoring method, such as alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase, gamma glutamyl transferase, ammonia amylase and creatine kinase. Some metabolite enzyme assay items, such as hexokinase assay, glucose and urease coupling assay, are also available for continuous monitoring. 4. turbidimetry turbidimetric method can be used for determination of turbidity reaction projects, mostly of immunoturbidimetry, apolipoprotein, immunoglobulin, complement, anti O, rheumatoid factor, and other proteins in serum such as albumin, haptoglobin and transferrin are available this method. Two. Analysis of parameter settings The general operating procedures of the analyzer include sampling, flushing, absorbance detection, data processing, etc.,