第十三章 可见分光光度法和紫外分光光度法(国外英文资料).doc

第十三章 可见分光光度法和紫外分光光度法(国外英文资料) The thirteenth chapter is visible spectrophotometry and ultraviolet spectrophotometry Home page Problem analysis Student self test questions Student answer Chapter after Exercises answer Problem analysis [TOP] Two cases of 13-1 with ortho phenanthroline photometric method for the determination of iron concentration in the sample, known for its approximately 5? 10-7 G? ML-1, this time? The molar absorption coefficient of 1.1? 104 L? Mol -1? Cm-1, asked to make the determination of the absorbance at 0.2 ~ 0.3, the selection of thick color pan (mol-1 Fe = 55.85 G?) Solution, when C is expressed in mol, L-1 A= epsilon B C The known concentration is 5 * 10-7 g, mL-1 be L When A = 0.2, When A = 0.3, Example 13-2 A=0.400 of a coloured solution in a 1cm cuvette. Dilute the solution to half of its original concentration and transfer to a 3 cm cuvette. Calculate the A and T values at the same wavelength. B1=1 cm, b2=3, cm, A1=0.400, c2=0.5, C1 A1= epsilon B1 C1, A2= epsilon B2 C2 Get A2=0.60 T=10-A2=10-0.6=25% The relative molecular mass of the amine sample cases of 13-3 is unknown, by using picric acid (Mr = 229) after processing into amine picrate (1:1 addition compound). When the wavelength of 380 nm, amine picrate Moore in 95% ethanol absorption coefficient was 1.35 * 104 L? Mol 1? Cm 1. The 0.0300 g amine picrate dissolved in 95% ethanol, accurately prepared 1 L solution at 380 nm, B = 1 cm, the measured absorbance A = 0.800, try to calculate the unknown molecular weight amine. The relative molecular mass of the unknown amine was X. Because of the formation of amine and picric acid amine picrate 1:1 additive compound, so the relative molecular mass of amine salt of picric acid (for 229x) Substituting V = 1.00L, M = 229 + X R x = 227 g mol-1? Example 13-4 phosphorus in urine can be treated with molybdate, and then molybdenum blue is formed with amino naphthol sulfonic acid. Spectrophotometry is carried out at 690 nm wavelength. A patient 24h