鞘内注射抗促肾上腺皮质激素释放激素血清对针刺夹脊穴镇痛影响及机制的研究.pdf

The Role of Intrathecally Administered Anti-CRH Serum in the Analgesic Effects of Acupuncture at Jiaji Acupoints Specialty: Science of Acupuncture, Moxibustion and Chinese Massage Author: Chen Xinyong Tutor: Prof. Wu Fudong Abstract Objective: To explore the mechanism of endogenous corticotropin-releasing hormone (CRH) involvement in acupuncture analgesia and neuro-endocrine-immunity (NEI) regulation, by observing the influence of intrathecally (icv) administered anti-CRH serum(ACRHS) on the analgesic and NEI regulatory effects of electro-acupuncture (EA) at EX-B2 in CFA-induced arthritis inflammatory pain model （AA ）rats; to preliminarily probe the cellular signal transduction mechanism of endogenous CRH in its involvement in acupuncture analgesia, by studying the expression changes of inhibitory GTP-binding protein (Gi) in hypothalamus after EA and intrathecal ACRHS injection in AA rats; to investigate the relationship between endogenous CRH and central 5-hydroxytryptamine (5-HT), by observing the influences of administrated icv anti-CRH serum and anti-5-HT serum on the pain threshold of AA rats as well as on the analgesic roles of EA in these rats. Methods: AA rats were used as the subjects of the study. By using methods of pain behavior observation, foot cubage measurement, radioimmunoassay and MTT, we observed the roles of EA at EX-B2 and intrathecal ACRHS injection on the pain threshold, foot cubage, the contents of 5-HT in hypothalamus ，the contents of β-endorphin in hypothalamus and spinal cord, the level of IL-6 and TNF-α in blood serum of AA rats. Both the expression of CRH and Gi in hypothal