销售管理菝葜水提物对高脂饮食诱导肥胖小鼠体重和脂肪代谢的影响.pdfVIP

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.2 菝葜水提物对高脂饮食诱导肥胖小鼠体重和 脂肪代谢的影响 潘永芳，郑国栋，张清峰，黎冬明 （江西农业大学食品科学与工程学院，江西省天然产物与功能食品重点实验室，江西南昌 330045） 摘要：研究菝葜水提物对高脂饮食诱导肥胖小鼠体重和脂肪代谢的影响。50 只ICR 雌性小鼠随机分成5 组，分别喂养基础饲料， 高脂饲料和添加了不同剂量菝葜水提物的高脂饲料，定期测定体重和摄食量，投喂8 周后测定小鼠体重、肝脏和腹腔内脂肪组织（IPAT ） 重量，分析血清中生化指标和肝脏中脂肪代谢相关酶的活性。与高脂组相比，低、中和高剂量的菝葜水提物能显著的控制小鼠体重增 加、IPAT 重量和血清中GLU 和TG 浓度，体重增加分别减少了31.97%、38.31%和36.90%，IPAT 重量分别减少41.34% 、48.58%和 43.41%，GLU 浓度分别降低了28.50% 、17.15%和15.10%，TG 浓度分别降低35.86%、29.31% 、46.90% ；肝脏中ACO 的活性分别提 升了25.49% 、29.41%和26.47%，中、高剂量组AMPK 活性显著提升分别提升了29.17%、33.33%。以上结果表明，菝葜水提物抑制 小鼠体内脂肪沉积和减少体重增加主要是通过提升肝脏中脂肪氧化酶的活性，促进脂肪酸 β-氧化来实现，对脂肪合成进程无明显影 响。 关键词：菝葜水提物；体重；脂肪沉积；脂肪代谢相关酶活性 文章篇号：1673-9078(2014)2-12-16 Effects of Water Extract from Smilax china on Body Weight and fat Metabolism of High-fat Diet Induced Obese Mice PAN Yong-fang, ZHENG Guo-dong, ZHANG Qing-feng, LI Dong-ming (Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China) Abstract: Fifty female ICR mice were randomly assigned to five groups, individually fed on commercial diets ，high-fat diet, high-fat diet with low, middle, high level of water extraction from Smilax china (WESC) for 8 weeks,. Body weight and food intake were inspected regularly during this period; after eight weeks, the weights of body, liver and intraperitoneal adipose tissue (IPAT) were measured, and the serum index and the activities of liver lipid metabolism related enzymes in mice were analyzed. Compared with the high-fat group, low, middle and high doses of WESC significantly decreased body weight gain, IPAT weight and the concentrations of GLU and TC in serum. The body weight gain decreas