色谱故障及维说明.pptVIP

HPLC Column and System Troubleshooting 色谱柱和色谱系统的故障检修;HPLC Components HPLC 的组成;Categories of Column Problems 色谱柱问题的类型;Pressure Issues压力问题 ;Determining the Cause and Correcting High Back Pressure 查出原因 纠正压力;Column Cleaning 柱子清洗;Column Cleaning柱子清洗;How to Change a Frit 怎样更换滤头;Preventing Back Pressure Problems压力问题的预防;Preventing Back Pressure Problems:In-Line Devices 压力问题的预防:在线装置;Preventing Back Pressure Problems: Sample Preparation压力问题的预防:样品制备;Peak Shape Issues 峰形问题;Split Peaks 裂峰;Split Peaks 裂峰Column Contamination 柱污染;Split Peaks 裂峰Injection Solvent Effects 溶剂化效应;Determining the Cause of Split Peaks 裂峰原因的确定;Peak Tailing, Broadening and Loss of Efficiency峰拖尾、变宽和柱效降低;Peak Tailing Column “Secondary Interactions”峰拖尾柱“??级效应”;Peak Tailing Column “Secondary Interactions”峰拖尾柱“次级效应”;Peak Tailing 峰拖尾Column Contamination 柱污染;Peak Tailing/Broadening Sample Load Effects峰拖尾/变宽样品过载效应;Peak Broadening,Splitting Column Void 峰变宽,裂开 柱塌陷;Broad Peaks Unknown “Phantom” Peaks;Peak Tailing峰拖尾 Injector Seal Failure进样器密封性差;Peak Tailing 峰拖尾Extra-Column Volume 柱外死体积效应;Determining the Cause of Peak Tailing峰拖尾原因的确定;Retention Issues保留时间问题;Changes in Retention Same Column, Over Time相同柱子上保留时间改变,超时;Mobile phase Change Causes Change in Retention流动相改变引起保留时间改变;Column Aging/Equilibration Causes Retention/Selectivity Changes柱老化／平衡不够引起保留时间／选择性改变;Determining the Cause of Retention Changes on the same Column同一柱上保留时间改变原因的确定;Change in Retention/Selectivity Column-to Column柱与柱之间保留时间/选择性的改变;Lot-to-Lot Selectivity Change (pH) 批与批之间选择性改变(pH);Conclusions 结论