宣威地区肺腺癌病人肺组织特异miRNAs表达谱与靶基因及其.PDFVIP

·238 · doi 10.3969/j.issn. 1673-4254.2017.02.16 J South Med Univ, 2017, 37(2): 238-244 临床研究 宣威地区肺腺癌病人肺组织特异宣威地区肺腺癌病人肺组织特异miRNAsmiRNAs 表达谱和靶基因及其表达谱和靶基因及其 信号信号通路预测通路预测 陈 帅，周永春，陈 颖，陈小波，李光剑，杨加鹏，雷玉洁，赵光强，黄秋博，杨长绍，杜亚茜，黄云超 昆明医科大学第三附属医院//云南省肿瘤医院//云南省肺癌研究重点实验室，云南 昆明 650000 摘要：目的筛选出宣威地区肺腺癌病人肺组织miRNAs 差异表达谱,预测其相关靶基因和信号通路。方法 选取来自宣威地区 肺腺癌24 例和非宣威地区肺腺癌10例患者手术切除的肺癌组织和正常肺组织标本，miRNAs 芯片检测34对肺癌切除标本，筛 选宣威肺腺癌miRNAs 差异表达谱，AGO 分析和KEGG Pathway 分析预测其miRNAs 的生物与肺癌相关的靶基因和信号通 路。结果 miRNAs 芯片检测：与非宣威肺腺癌比较，宣威肺腺癌差异表达显著的特异miRNAs 34个：表达上调的miRNAs 有23 个，表达下调的miRNAs 有11个。AGO 富集分析及KEGG 数据库映射分析发现：预测主要集中在PI3K/Alt 信号通路附近，其预 测靶基因有：GF、RTK、SOS、IRS 1、BCAP、CYTOKINSR、ECM、ITGB、FAK 和GβY ，可能对肺癌生物学功能产生影响的靶基因 主要集中在PI3K/Alt ，WNT 和MAPK 通路。结论 筛选出这些特异性miRNAs 可能在宣威肺腺癌癌变和进展中起重要作用，预 测的靶基因可能与调节PI3K/Alt ，WNT 和MAPK 信号通路的作用有关。 关键词：miRNA 芯片；miRNAs ；宣威肺腺癌；靶基因；信号通路 Specific microRNA expression profiles of lung adenocarcinoma in Xuanwei region and bioinformatic analysis for predicting their target genes and related signaling pathways CHEN Shuai, ZHOU Yongchun, CHEN Ying, CHEN Xiaobo, LI Guangjian, YANG Jiapeng, LEI Yujie, ZHAO Guangqiang, HUANG Qiubo, YANG Changshao, DU Yaxi, HUANG Yunchao Department of Thoracic Surgery, Third Affiliated Hospital of Kunming Medical University/ Tumor Hospital of Yunnan Province/ Yunnan Key Laboratory of Lung Cancer Research, Kunming 650000, China Abstract: Objective To identify differentially expressed microRNAs (miRNAs) related to lung adenocarcinoma in Xuanwei region and predict their target genes and related signaling pathways based on bioinformatic analysis. Methods High-throughput microarray assay was performed to detect miRNA expression profiles in 34 paired human lung adenocarcinoma and adjacent normal tissues (including 24 cases in Xuanwei region and 10 in other regions). Gene ontology and KEGG pathway analyses were used to predict