药材及资源菘蓝rDNA及端粒多色荧光原位杂交分析.PDFVIP

• 972 • 中草药 Chinese Traditional and Herbal Drugs 第 42 卷 第 5 期 2011 年 5 月 • 药材与资源 • 菘蓝 rDNA 及端粒多色荧光原位杂交分析 1 2* 杨 飞 ，李立家 1. 长江大学医学院 分子生物学部，湖北 荆州 434023 2. 武汉大学生命科学学院，植物发育生物学教育部重点实验室，湖北 武汉 430072 摘 要：目的 对菘蓝 45S rDNA 、5S rDNA 和端粒进行物理定位，并分析其核型。方法 使用多色荧光原位杂交技术 （FISH ） 进行定位。结果 菘蓝基因组有一对 45S rDNA 位点位于 4 号染色体，一对 5S rDNA 位点位于 5 号染色体，14 对端粒信号 位于染色体两端；核型公式为 2n ＝2x ＝14＝10m （2SAT ）＋4sm ，属 2A 核型。结论 为菘蓝核型分析、分子细胞遗传图谱 构建提供了稳定、有效的细胞学标记。 关键词：菘蓝；rDNA ；端粒；荧光原位杂交；核型 中图分类号：R282.12 文献标志码：A 文章编号：0253 - 2670(2011)05 - 0972 - 04 Multicolor fluorescence in situ hybridization analysis on rDNA and telomere of Isatis indigotica 1 2 YANG Fei , LI Li-jia 1. Department of Molecular Biology, College of Medicine, Yangtze University, Jingzhou 434023, China 2. Key Laboratory of the MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China Abstract: Objective To investigate the physical location of 45S, 5S rDNA, and telomere sequences and analyze the karyotype of the metaphase chromosomes of Isatis indigotica . Methods Multicolor fluorescence in situ hybridization (FISH) approach was used to detect the physical location of the above sequences. Results One pair of 45S rDNA sites located on chromosome 4, one pair of 5S rDNA sites located on chromosome 5 and 14 pairs of telomere sites located at the ends of each chromosome in I. indigotica were detected. The karyotype formula is 2n = 2x = 14 = 10m (2SAT) + 4sm, which belongs to 2A karyotype. Conclusion This research provides effective cytological markers for karyotype analysis and construction of a molecular cytogenetic map of I. indigotica . Key words: Isatis indigotica Fort.; rDNA; telomere; fluorescence in situ hybridization (FISH); karyotype 菘蓝 Isatis indigotica