脑缺血和脑缺血再灌注损伤动物模型制备方法和评价.PDFVIP

第 1 卷第3 期 神经药理学报 Vol. 1 No. 3 2011 年6 月 Acta Neuropharmacologica June. 2011 脑缺血及脑缺血再灌注损伤动物模型 制备方法及评价 王  树  张  力 河北北方学院药学系，张家口，075000 ，中国 【摘要】脑血管病是临床常见病、多发病，选择和应用较为可靠的脑血管病动物模型对其预防和治疗的研究显 得尤为重要。本文对常见的各种动物全脑缺血和局部脑缺血及再灌注动物模型的种类、制作方法和特点作了详 细分类及介绍，特别详述了改良Himori 法（暂时性阻断两侧颈总动脉制备小鼠脑缺血再灌注模型）及该模型制 作的关键步骤。通过对现有动物模型应用特点的评价，为选择合适的脑缺血及脑缺血再灌注动物模型研究脑血 管病，具有重要指导意义。 【关键词】脑缺血；脑缺血再灌注；动物模型；改良Himori 法；制备方法；评价 【中图分类号】R743.3 【文献标识码】A 文章编号：2095-1396 （20 11）03-0031-010 Methods and Evaluation of Animal Models of Cerebral Ischemia and Cerebral Ischemia Reperfusion Injury WANG Shu ，ZHANG Li Department of Pharmacology ，Hebei North University ，Zhangj iakou ，075000 ，China 【ABSTRACT 】Cerebrovascular disease is a clinically common disease and frequently-occur- ring disease. The selection and application of reliable animal models are particularly important in studies for the prevention and treatment of cerebrovascular disease. The types ，operation proce- dures of models ，and characteristics of the global cerebral and local cerebral ischemia and cere- bral ischemia reperfusion inj ury in different animal models were discussed in detail in this paper. The improved Himori method （the model of cerebral ischemia reperfusion was produced in mice by temporarily obstructing bilateral common carotid arteries）and some key steps were especially presented in detail. The evaluation of the application characteristics of existing animal models will help to select the appropriate models of cerebral ischemia and cerebral ischemia reperfusion for studying cere