酪酸梭菌对高尿酸血症大鼠血尿酸和炎性因子水平的影响.PDFVIP

·678 · doi 10.3969/j.issn. 1673-4254.2017.05.19 J South Med Univ, 2017, 37(5): 678-682 基础研究 酪酸梭菌对高尿酸血症大鼠血尿酸及炎性因子水平的影响酪酸梭菌对高尿酸血症大鼠血尿酸及炎性因子水平的影响 王 力，方志荣，沈雅庭，刘彦波，刘丽丽 中国人民解放军第413 医院内二科，浙江 舟山 316000 摘要：目的研究酪酸梭菌干预对高尿酸血症大鼠血清尿酸、脂多糖、白介素6、肿瘤坏死因子α的影响。方法 将40 只SD大鼠随 7 CFU/d），每组10只。除正常 机分为正常对照组、高尿酸模型组、苯溴马隆干预组（3 mg/kg ·d）和酪酸杆菌干预组（活菌1.5×10 对照组外各组给予酵母膏联合氧嗪酸钾灌胃制备高尿酸血症模型，各组药物及活菌每天灌胃1次，连续12周。观察各组大鼠血 尿酸、脂多糖、白介素6、肿瘤坏死因子α水平变化。结果 实验期间高嘌呤饮食大鼠血尿酸水平较正常对照组明显升高（P0.01）， 高尿酸血症模型建立成功且稳定。高尿酸模型组大鼠血脂多糖、白介素6、肿瘤坏死因子α水平与血尿酸水平呈正相关。苯溴马 隆及酪酸梭菌干预处理后大鼠血尿酸、脂多糖、白介素6、肿瘤坏死因子α水平有明显下降（P0.01）, 干预时间越长，效果越佳，其 中苯溴马隆降尿酸效应更为明显，酪酸梭菌下调脂多糖、白介素6、肿瘤坏死因子α等炎性因子作用更为明显（P0.01）。结论 高 尿酸血症大鼠可能存在慢性炎症反应，酪酸梭菌可有效降低血尿酸水平，同时抑制炎症细胞因子的生成，维持肠道免疫稳态。 关键词：酪酸梭菌；高尿酸血症；大鼠；炎性介质 Effects of Clostridium butyricum on serum uric acid and inflammatory mediators in rats with hyperuricemia WANG Li, FANG Zhirong, SHEN Yating, LIU Yanbo, LIU Lili Department of Internal Medicine, 413 Hospital of PLA, Zhoushan 316000, China Abstract: Objective To investigate the effects of intragastric administration of Clostridium butyricum in regulating serum uric acid, lipopolysaccharides (LPS), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in rats with hyperuricemia rats. Methods Forty SD rats were randomized into 4 equal groups, namely the normal control group, hyperuricemia model group, benzbromarone (3 mg/kg daily) intervention group and live Clostridium butyricum group (1.5×107 CFU/day). Except for those in the control group, the rats were subjected to intragastric administration of yeast extract and oteracil potassium once daily for 12 weeks to induce hyperuricemia with corresponding treatments. The changes in serum uric acid, lipopolysaccharides , IL-6 and TNF-α in each group were detected. Results The serum level of uric acid