不同栽培制度对黄瓜连作土壤微生物群落结构的影响.pdfVIP

中国蔬菜 2012（8）：26-31 CHINA VEGETABLES 不同栽培制度对黄瓜连作土壤微生物群落 结构的影响 1 1，2 1* 王旭晖 张雪艳 高丽红 1 2 （ 中国农业大学农学与生物技术学院，北京 100193； 宁夏大学农学院，宁夏银川 750021） 摘 要：采用群落水平碳源利用（Biolog）与变性梯度凝胶电泳（PCR-DGGE）技术研究不同栽培制 度对黄瓜根际土壤微生物功能多样性和细菌群落结构的影响。Biolog 结果显示：在秋冬茬，各个栽培制度 与对照相比均提高了土壤微生物功能多样性和均匀度；主成分分析表明：青蒜处理与对照相比碳源利用 特征在 3 个茬口均出现差异；填闲茼蒿和填闲普通白菜（小白菜）处理土壤微生物碳源利用特征相似； DGGE 结果表明：夏季填闲茼蒿和普通白菜，可以增加土壤细菌种类和多态性。 关键词：黄瓜；栽培制度；Biolog；DGGE 中图分类号：S642.2 文献标识码：A 文章编号： 1000-6346（2012）08-0026-06 Effects of Different Cultural Systems on Cucumber Soil Microbial Community Functional Diversity and Bacteria Communities 1 1，2 1* WANG Xu-hui ，ZHANG Xue-yan ，GAO Li-hong 1 2 （College of Agronomy and Biotechnology ，China Agricultural University ，Beijing 100193 ，China； Agronomy College ，Ningxia University ，Yinchuan 750021，Ningxia ，China ） Abstract ：Using Biolog and PCR-DGGE to study the effects of different cultural systems on cucumber（Cucumis sativus L.）soil microbial community functional diversity and bacteria communities of cucumber rhizosphere soil．The results of Biolog showed that during the autumn winter season，all treatments improved the functional diversity and uniformity of soil microbial．The principal component analysis showed that carbon utilization by soil microbial communities of catching garlic have greatly changed in all seasons；catching crown daisy and pakchoi have the similar carbon utilization．The results of DGGE showed that introduced several catch crops in summer could increase the bacteria species and polymorphism includi